Identify and characterize palmitylated proteins with Click-iT® palmitic acid, azide using the powerful click chemistry, a simple and robust two-step labeling and detection technique. In step one, the azide-containing biomolecule is fed to cells or animals and actively incorporated into proteins. Unlike other labels such as biotin or a fluorescent dye, the azide-tag is small enough that the tagged molecule is an acceptable substrate for the enzymes that incorporate this building block into proteins. Detection utilizes the chemoselective ligation or “click" reaction between and azide and an alkyne where the modified protein is detected with the corresponding alkyne-containing dye or hapten using either the Click-iT® Cell Reaction Buffer Kit or the Click-iT® Protein Buffer Kit. With the Click-iT® Cell Reaction Buffer Kit, cells can be analyzed by fluorescence microscopy, flow cytometry or high-content imaging and analysis (HCS) together with other biomarkers of interest for content and context rich results. With the Click-iT® Protein Reaction Buffer Kit, achieve detection sensitivity in 1-D gels and western blots in the low femtomole range or perform LC-MS⁄MS and MALDI MS analysis.
For Research Use Only. Not for use in diagnostic procedures.
사양
그린 기능
Less hazardous
제품 유형
Palmitic Acid
수량
1 mg
라벨링 방법
Metabolic Labeling
배송 조건
Room Temperature
제품라인
Click-iT™, Molecular Probes™
라벨 또는 염료
Azide
형식
Solid
Labeling Target
Proteins, Proteins, Proteins
구성 및 보관
Store at ≤-20°C, desiccated and protected from light.