Calcein AM, cell-permeant green and blue dyes
Calcein AM, cell-permeant green and blue dyes
Invitrogen™

Calcein AM, cell-permeant green and blue dyes

Calcein AM은 대부분의 진핵 세포에서 세포 생존율을 측정하는 데 사용할 수 있는 세포 투과성 dye입니다. 살아있는 세포에서 비형광 calcein AM은자세히 알아보기
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카탈로그 번호수량ProductTypeSpecs
C14291 mg
C3100MP20 x 50 μg
C14301 mg
C30991 mL
C3485220 x 50 μg
C481100 mg
카탈로그 번호 C1429
제품 가격(KRW)
246,000
Online offer
Ends: 30-Sep-2025
289,000
할인액 43,000 (15%)
Each
재고 준비 예상일 23-Jul-2025
카트에 추가하기
수량:
1 mg
제품 가격(KRW)
246,000
Online offer
Ends: 30-Sep-2025
289,000
할인액 43,000 (15%)
Each
카트에 추가하기
Ask our AI about this Product
Calcein AM은 대부분의 진핵 세포에서 세포 생존율을 측정하는 데 사용할 수 있는 세포 투과성 dye입니다. 살아있는 세포에서 비형광 calcein AM은 세포간 esterase에 의해 acetoxymethyl ester 가수분해 후 녹색 형광 calcein으로 전환됩니다. 이 염료는 자사의 특수 패키지(C-3100)에도 들어있으며 DMSO(C-3099)에 녹일 수 있습니다. 파장 길이가 긴 dye 로는 새로운 CellTrace calcein red-orange AM (C-34851)을 확인하십시오.

본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
세포 투과성Cell-permeant
염료 유형Other Label(s) or Dye(s)
여기/방출322/437 nm
분자량465.41
수량1 mg
시약 유형Cell Tracker Compounds, Cell Labeling Reagents
배송 조건Room Temperature
타겟 효소Esterase
Emission449
용도(애플리케이션)Cell Tracing, Cell Tracker
용도 (장비)Fluorescence Microscope
제품 유형Calcein Blue, AM
Unit SizeEach
구성 및 보관
Store in freezer (-5°C to -30°C) and protect from light.
PG2624-PJT8477-COL022341-Media-Promo-Pod-Graphic-Global-F-278x123
four-plex-image-COS7-live-cell zoomed
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그림

형광 스펙트럼

Fluorescence spectra

문서 및 다운로드

Certificates | 증명서

Lot 번호Certificate TypeDateCatalog Number(s)
3143107Certificate of Analysis2025년 4월 08일C3100MP
3101991Certificate of Analysis2025년 3월 26일C1430
2994045Certificate of Analysis2024년 9월 25일C1429
2983198Certificate of Analysis2024년 8월 05일C3100MP
2978444Certificate of Analysis2024년 7월 21일C481
시험 성적서 관련 5 개의 결과가 검색되었습니다.

Safety Data Sheets | 물질 안전 보건 자료

Product Information

자주 묻는 질문(FAQ)

Calcein AM, a green dye, is typically used as a general cytoplasmic stain, but not recommended with GFP-positive cells. For GFP-expressing cells there are other colors available: Calcein Blue AM, Calcein Violet AM, and Calcein Red-Orange AM. The retention time of these dyes in live cells is dependent upon the inherent properties of the cell.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

One possibility is that there is spectral bleedthrough between the dyes. Be sure to check the single-color samples by imaging the red cells in green and imaging the green cells in red, using the optimal imaging settings for the other color. If you see bleedthrough with these controls, then you will have to reduce the dye label concentration to reduce the brightness of the dyes, or choose dyes that are farther apart spectrally. If the issue isn’t bleedthrough, another possibility is that the cells were not adequately washed after staining, allowing some unincorporated dye to remain and label the other cells after they were introduced. Extending washes and wash times should help with this.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Calcein, AM diffuses into cells, the 'AM' moiety is cleaved by cellular esterases, and then the dye molecules are observed in the cytoplasm without binding to anything. This gives a 'whole cell' stain. It also means that the dyes are not crosslinked with aldehyde-based fixation and thus will be lost upon fixation. Additionally, any disruption of plasma membrane, such as with detergents or trypsinization, will lead to leakage of the dye from the cell.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

First, make sure you aren’t staining in the presence of serum, since serum can have esterase activity that can prematurely cleave the AM group on these dyes, preventing entry into cells. After staining, it’s okay to return the cells to medium containing serum. After this, you can try increasing the concentration and label time to get a higher intensity.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Calcein dyes diffuse into cells, the 'AM' moiety is cleaved by cellular esterases and then are observed in the cytoplasm without binding to anything. This provides a 'whole cell' label. Calcein dyes may be pumped out by normal cellular efflux mechanisms, sometimes within a very short time, especially for cell types that may exhibit drug resistance, unless the efflux is inhibited (such as with probenecid). The dyes are not crosslinked with aldehyde-based fixation, unlike protein-binding CellTracker dyes, and thus will be lost upon fixation. Additionally, any disruption of plasma membrane, such as with detergents or trypsinization, will lead to leakage of the dyes from the cell.

Find additional tips, troubleshooting help, and resources within our Cell Tracing and Tracking Support Center.

인용 및 참조 문헌 (1025)

인용 및 참조 문헌
Abstract
Nitric oxide mediates natural polyphenol-induced Bcl-2 down-regulation and activation of cell death in metastatic B16 melanoma.
Authors:Ferrer P,Asensi M,Priego S,Benlloch M,Mena S,Ortega A,Obrador E,Esteve JM,Estrela JM
Journal:The Journal of biological chemistry
PubMed ID:17135264
Cloning and expression of murine sister of P-glycoprotein reveals a more discriminating transporter than MDR1/P-glycoprotein.
Authors:Lecureur V,Sun D,Hargrove P,Schuetz EG,Kim RB,Lan LB,Schuetz JD
Journal:Molecular pharmacology
PubMed ID:10617675
Membrane permeabilization induced by cytolytic delta-endotoxin CytA from Bacillus thuringiensis var. israelensis.
Authors:Butko P,Huang F,Pusztai-Carey M,Surewicz WK
Journal:Biochemistry
PubMed ID:8784190
Deposition of laminin 5 by keratinocytes regulates integrin adhesion and signaling.
Authors:Nguyen BP,Gil SG,Carter WG
Journal:The Journal of biological chemistry
PubMed ID:10926936
Large-scale chemical dissection of mitochondrial function.
Authors:Wagner BK,Kitami T,Gilbert TJ,Peck D,Ramanathan A,Schreiber SL,Golub TR,Mootha VK
Journal:Nature biotechnology
PubMed ID:18297058
Mitochondrial oxidative phosphorylation (OXPHOS) is central to physiology and disease pathogenesis. To systematically investigate its activity and regulation, we performed a wide range of assays of OXPHOS physiology and nuclear and mitochondrial gene expression across 2490 chemical perturbations in muscle cells. Through mining of the resulting compendium, we discovered that: ... More
1025 total citations

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