Thermo Scientific BL21(DE3) Competent Cells are suitable for the expression of non-toxic heterologous genes. The strain contains the lambda DE3 prophage that carries the gene for T7 RNA polymerase under control of a lacUV5 promoter, allowing expression of the T7 RNA polymerase to be induced with IPTG. BL21(DE3) is an E. coli B strain and does not contain the lon protease. It is also deficient in the outer membrane protease OmpT. The lack of these two key proteases reduces degradation of heterologous proteins expressed in the cells.
• High transformation efficiency: >1x107 cfu/μg pUC19 DNA • Specifically constructed for high-level expression of recombinant proteins • Genetic markers that minimize RNA and protein degradation • Convenient two reactions per vial packaging • S.O.C. Outgrowth medium included
Applications BL21(DE3) Competent Cells are suitable for high-level T7 expression of recombinant proteins.
Genotype F– ompT hsdSB (rB–, mB–) gal dcm (DE3)
Note: Not suitable for maintenance of expression plasmids. Use DH10B or DH5α competent cells that do not carry the gene for T7 RNA polymerase for plasmid DNA propagation (Cat. Nos. EC0112, EC0113).
For Research Use Only. Not for use in diagnostic procedures.
사양
제품 유형
Competent Cell
효율
High Efficiency (>1x10^7 cfu/μg pUC19 DNA)
세포 유형
Chemically Competent
메틸화 DNA 클로닝
Yes
박테리아 또는 효모 균주
BL21(DE3)
블루/화이트 스크리닝
No
배송 조건
Dry Ice
종
E. coli
프로모터
LacUV5
형식
Tube
제품라인
Thermo Scientific™
수량
10 x 100 μL
구성 및 보관
• 10 x BL21(DE3) Competent Cells (100 μL) • pUC19 DNA (50 μL) (10 pg/μL) • S.O.C. Medium (5 mL)