2D electrophoresis is used to separate proteins based upon their molecular weight after isoelectric focusing (IEF). 2D gels are generally designed to accept IEF tube gels, sample lanes sliced from vertical or horizontal IEF slab gels, or immobilized pH gradient (IPG) strips. Gels are usually run under denaturing conditions using SDS in the gel running buffers. ZOOM™ Gels are specifically designed for 2D separation of proteins that have been first separated by their pI on 7-cm IPG strips (e.g., ZOOM™ Strips). The IPG well of ZOOM™ Gels accommodates the full length of a 7-cm IPG strip (Figure 1). In addition to the IPG well, ZOOM™ Gels have a separate well designed for use with a molecular weight standard. The ZOOM™ cassette is the standard 10 cm x 10 cm format. ZOOM™ Gels are available in NuPAGE™ and Novex™ Tris-Glycine gel formulations.
For Research Use Only. Not for use in diagnostic procedures.
사양
용도 (장비)
XCell SureLock Mini-Cell, ZOOM IPGRunner Mini
젤 비율
4 to 20%
젤 크기
Mini
Gel Thickness
1.0 mm
젤 유형
Tris-Glycine
제품 유형
Gradient Page
샘플 로딩 부피
7 cm strips
다음의 분리
Protein
분리 범위
20 to 200 kDa
분리 유형
Denaturing
유통 기한
8 Weeks
웰
1-well
분리 모드
Molecular Weight
제품라인
Novex™, ZOOM™
수량
Each
배송 조건
Wet Ice
시스템 유형
ZOOM™
두께
1.0 mm
웰 디자인
IPG Well
길이(미터법)
8 cm
길이(미터법) 젤
8 cm
폭(미터법)
8 cm
폭(미터법) 젤
8 cm
구성 및 보관
One box contains 10 gels. Store in refrigerator (2–8°C). Do not freeze. Shelf life is 8 weeks.