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Phusion™ High-Fidelity DNA Polymerases (2 U/μL)
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
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보기 방식 변경
| 카탈로그 번호 | 색상 | 반응 수 |
|---|---|---|
| F-530XL | Colorless | 2000 Reactions |
| F530S | Colorless | 100 Reactions |
| F530L | Colorless | 500 Reactions |
| F534S | Green | 100 Reactions |
| F534L | Green | 500 Reactions |
카탈로그 번호 F-530XL
제품 가격(KRW)
2,603,000
Online offer
Ends: 30-Sep-2025
2,892,000할인액 289,000 (10%)
Each
색상:
Colorless
반응 수:
2000 Reactions
제품 가격(KRW)
2,603,000
Online offer
Ends: 30-Sep-2025
2,892,000할인액 289,000 (10%)
Each
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is an excellent choice for cloning and other applications requiring high fidelity. Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerases.
Features of Phusion™ High-Fidelity DNA Polymerase include:
- High fidelity (52X Taq)
- Fast PCR due to short extension times (15–30 s/kb)
- Robust performance, minimal optimization needed
- High yields of PCR products with minimal enzyme amounts
- Available in Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)
Applications
- High-fidelity PCR
- Cloning
- Template generation for sequencing
- Amplification of difficult (GC-rich) templates
- Long-range PCR (up to 20 kb)
- Mutagenesis
- High throughput PCR
- Microarray
Note: Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our convenient Tm calculator.
For Research Use Only. Not for use in diagnostic procedures.
사양
색상Colorless
농도2 U/μL
Fidelity (Taq 대비)52X
핫 스타트No
반응 수2000 Reactions
오버행Blunt
중합효소Phusion High Fidelity DNA Polymerase
제품 유형High-Fidelity DNA Polymerase
수량2000 units
반응 형식Standalone
배송 조건Dry Ice
크기(최종 제품)20 kb or less
용도(애플리케이션)Standard PCR, High-fidelity PCR
GC-Rich PCR PerformanceHigh
반응 속도Fast
Unit SizeEach
구성 및 보관
• Phusion DNA Polymerase (2 U/μL)
• 5X Phusion HF & GC Buffers
• DMSO
• 50 mM MgCl2 solution
Phusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2 in the final 1X concentration.
Store at -20°C.
• 5X Phusion HF & GC Buffers
• DMSO
• 50 mM MgCl2 solution
Phusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2 in the final 1X concentration.
Store at -20°C.