The FIX & PERM™ Cell Permeabilization Kit is used in flow cytometry for fixing and permeabilizing cells in suspension, allowing access of antibody to intracellular targets without sacrificing scatter characteristics.



• Flexible—compatible with multiple sample types and cellular antigens

• Robust—scatter pattern of cells unaffected

• Efficient workflow—simultaneous detection of intracellular and surface markers



The FIX & PERM™ Cell Permeabilization Kit is suitable for various biological samples and has been validated for use with cell lines, whole blood, and bone marrow. The FIX & PERM™ kit has been shown to accurately identify previously undetectable intracellular markers. Unlike other fixation and permeabilization reagents available on the market, the FIX & PERM™ staining protocol leaves the morphological scatter characteristics of the cells intact.



The FIX & PERM™ formula was specifically designed to reduce background staining and allow simultaneous addition of fluorochrome-labeled antibodies during the permeabilization step.



How It Works:

The FIX & PERM™ Cell Permeabilization Kit contains 2 reagents: Fixation Medium (Reagent A) and Permeabilization Medium (Reagent B). Cells are incubated for 15 minutes at room temperature with the fixation medium, washed, and then incubated with the permeabilization medium and fluorochrome-labeled antibody.



The FIX & PERM™ Cell Permeabilization Kit is available in two sizes (50 and 200 tests). Reagent A and Reagent B are also available separately in a bulk size (1000 tests).