The Tango™ EDG1-bla U2OS cells contain the human Endothelial Differentiation, Sphingolipid G-protein-coupled Receptor, 1 (EDG1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.
The Tango™ EDG1-bla U2OS cells cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ EDG1-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product.
For Research Use Only. Not for use in diagnostic procedures.
사양
등록 번호
NM_001400.2
어세이 입력
Cell-based beta-arrestin recruitment
셀 상태
Dividing Cells
세포질 후미
Native Tail
검출 방법
Fluorescent
유전자 기호
EDG1
개체군 상태
Single Cell Clone Origin
제품라인
Tango™
리포터 유전자
BLA (Beta-Lactamase)
타겟 입력
EDG1
유형
U2OS Cell
세포주
U2OS
System Type
Tango™
Druggable Target
GPCR
구성 및 보관
K1520 Tango™ EDG1-bla U2OS cells (LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: • Tango™ EDG1-bla U2OS cells • Tango™ EDG1-bla U2OS Assay Protocol • Certificate of Analysis
Storage: The Tango™ EDG1-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.