The S.N.A.P.™ MidiPrep Kit is a medium-scale plasmid DNA purification kit designed for fast and easy purification of pure plasmid DNA from 10-100 ml of overnight culture (Figure 1). The S.N.A.P.™ MidiPrep procedure takes just 40 minutes. You simply:
• Lyse cells and filter debris with the S.N.A.P.™ filtering column • Apply flow-through to the S.N.A.P.™ DNA binding column • Wash the column • Elute pure, supercoiled plasmid DNA in water or TE buffer
Plasmid DNA purified with the S.N.A.P.™ MidiPrep Kit is ready to use right off the column. No precipitation is necessary. Plasmid is eluted in TE or water at ∼200 ng/μl-perfect for subsequent applications including mammalian cell transfection. Typical yields are shown in Table 1.
For Research Use Only. Not for use in diagnostic procedures.
사양
형식
Kit
Isolation Technology
Spin Column
샘플 종류
Bacterial Culture
스케일
Midi
최종 제품 유형
Plasmid DNA
용도(애플리케이션)
Sequencing, Cloning, Transformation, Transfection, In Vitro Transcription
고처리량 호환성
Not High-throughput Compatible (Manual)
반응 수
20 Preps
프렙 스케일
100-200 μg (Medium-Scale) Plasmid DNA
제품라인
S.N.A.P. ™
제품 유형
Plasmid DNA MidiPrep Kit
수량
20 Preps
타겟
Plasmid DNA
테스트 시간
40 min.
구성 및 보관
Store the S.N.A.P.™ MidiPrep filtering columns, S.N.A.P.™ MidiPrep DNA binding columns, lysis buffer, binding buffer, wash buffer, and 4X final wash at room temperature. Store the resuspension buffer, precipitation salt, and RNase A at +4°C. All reagents are guaranteed stable for 6 months when properly stored.