The pGlow TOPO® TA Expression Kit uses the Cycle 3 GFP mutant (1) as a reporter protein for transcriptional activity. Cycle 3 GFP is ideal for non-invasive analysis of promoter elements within intact, living cells. The fluorescent property of Cycle 3 GFP allows in vivo detection in virtually any cell type or species using microscopy with wild-type GFP filter sets or by fluorescence-activated cell sorting methods.
For Research Use Only. Not for use in diagnostic procedures.
사양
배달 유형
Transfection
프로모터
None (Promoterless)
제품 유형
TOPO TA Expression Kit
리포터 유전자
GFP (Cycle 3)
선택 제제(진핵)
Geneticin™ (G-418)
단백질 태그
Untagged
클로닝 방법
TOPO-TA
벡터
pGlow, TOPO-TA Vectors
제품라인
TOPO™
용도(애플리케이션)
Reporter Assays, Clone your own Promoter
구성 및 보관
pGlow TOPO™ TA Expression Kits contains two boxes. The TOPO™ TA Cloning box contains linearized and topoisomerase I-activated pGlow-TOPO™ vector, dNTPs, primers for sequencing, salt solution, and control PCR template and primers; store at -20°C. The One Shot™ box contains One Shot™ TOP10 E. coli cells, S.O.C. medium, and a supercoiled control plasmid; store at -80°C. The kits are guaranteed stable for 6 months when properly stored.