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The FreeStyle MAX System is a complete system for mammalian transient protein production. This system combines Gibco FreeStyle media, FreeStyle MAX Reagent, and either FreeStyle CHO-S cells or FreeStyle 293 cells. The only equipment required to use the FreeStyle MAX System is a CO2 incubator and a platform shaker with shake flasks or a spinner culture.
The FreeStyle MAX System provides:
• An easy, rapid protocol for transient transfection of CHO and 293 cells in suspension cultures
• Up to milligram quantities of functional proteins with the expected post-translational modifications
• Simplified downstream purification of secreted proteins with FreeStyle™ serum-free medium
• Straightforward protocol for large-scale production
The FreeStyle MAX System can replace time-consuming stable cell line generation with large-scale functional protein production in one week.
The FreeStyle MAX System provides:
• An easy, rapid protocol for transient transfection of CHO and 293 cells in suspension cultures
• Up to milligram quantities of functional proteins with the expected post-translational modifications
• Simplified downstream purification of secreted proteins with FreeStyle™ serum-free medium
• Straightforward protocol for large-scale production
The FreeStyle MAX System can replace time-consuming stable cell line generation with large-scale functional protein production in one week.
For Research Use Only. Not for use in diagnostic procedures.
사양
세포주
FreeStyle™ CHO-S Cells
분류
Animal Origin-Free, Serum-Free
배달 유형
Transfection
배양 유형
Suspension Cell Culture
셀 유형
CHO
Transfection Technique
Lipid-based Transfection
발현 메커니즘
Cell-Based Expression
발현 시스템
Mammalian
용도(애플리케이션)
Protein Expression
주요 기능
Protein Production
제품라인
Freestyle™
제품 유형
Expression System
수량
1 Kit
구성 및 보관
Store FreeStyle cells in liquid nitrogen. Store FreeStyle MAX Reagent at 2–8°C. Store FreeStyle CHO Expression Medium, FreeStyle 293 Expression Medium, and OptiPRO SFM at 2–8°C in the dark. Store the pCMV SPORT at -20°C. Stability is guaranteed for 6 months under the recommended storage conditions. Please refer to the manual for additional storage information.
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