The LIVE/DEAD Cell Vitality Assay Kit provides a simple, two-color fluorescence assay that distinguishes metabolically active cells from injured cells and dead cells. The assay is based on the reduction of C₁₂-resazurin to red-fluorescent C₁₂-resorufin in metabolically active cells and on the uptake of the cell-impermeant, green-fluorescent nucleic acid stain, SYTOX Green dye, in cells with compromised plasma membranes (usually late apoptotic and necrotic cells). In this assay, dead cells emit mostly green fluorescence and healthy, metabolically active cells emit mostly red fluorescence; injured cells exhibit reduced red and green fluorescence.

View a selection guide for all Cell Viability Assays for Flow Cytometry.