AmpErase™ Uracil N-Glycosylase (UNG), part of the GeneAmp™ PCR Carry-over Prevention Kit, is a 26 kDa ultrapure, recombinant enzyme encoded자세히 알아보기
Have Questions?
카탈로그 번호 N8080096
제품 정가(KRW)/ 100 units
제품 정가283,000
온라인 행사241,000온라인 주문시에만 적용 (Supply Center 제외) .
(ends 01-Oct-2024)
공급가로그인계정에 따른 가격을 보시려면 로그인하십시오. 계정이 필요하십니까? 지금 바로 등록하십시오.
-
카트에 추가하기
대량 주문 또는 맞춤형 요청
제품 정가(KRW)/ 100 units
온라인 행사241,000온라인 주문시에만 적용 (Supply Center 제외) .
(ends 01-Oct-2024)
카트에 추가하기
AmpErase™ Uracil N-Glycosylase (UNG)
카탈로그 번호N8080096
제품 정가(KRW)/ 100 units
온라인 행사241,000온라인 주문시에만 적용 (Supply Center 제외) .
(ends 01-Oct-2024)
-
카트에 추가하기
AmpErase™ Uracil N-Glycosylase (UNG), part of the GeneAmp™ PCR Carry-over Prevention Kit, is a 26 kDa ultrapure, recombinant enzyme encoded by the E. coli uracil N-glycosylase gene, which has been inserted into an E. coli host to direct the expression of the wild type form of the enzyme. The enzyme removes any uracil incorporated into single- or double-stranded DNA.
GeneAmp™ PCR Carry-over Prevention Kit The GeneAmp™ PCR Carry-over Prevention Kit (available separately) provides reagents for a simple yet powerful method of ensuring that PCR products cannot be reamplified in subsequent PCR amplifications, thereby preventing false positive results. Features of this kit:
• Enzymatic method stops PCR carryover contamination, which prevents false positive results • Designed to degrade PCR products from previous PCR amplifications without degrading native nucleic acid templates, which improves amplification • No interference with any PCR or real-time PCR application • Optimized for use with GeneAmp™ PCR core reagents and GeneAmp™ instrument systems
Eliminate PCR carryover contamination The kit uses an enzymatic reaction analogous to the restriction-modification and excision-repair systems of cells to specifically degrade PCR products from previous PCR amplifications in which dUTP has been incorporated, without degrading native nucleic acid templates. The method used to render PCR products susceptible to degradation involves substituting dUTP for dTTP in the PCR mixture, and pretreating all subsequent PCR mixtures with AmpErase™ Uracil N-glycosylase (UNG) prior to PCR amplification. Products from PCR amplification contain uracil, and are readily distinguishable from native thymidine-containing DNA templates. Products from previous PCR amplifications are eliminated by excising uracil residues using UNG, and degrading the resulting abasic polynucleotide with heat. Although UNG is active on single- and double-stranded dU-containing DNA, ribouracil residues in RNA and dUTP itself are not substrates for UNG. AmpliTaq™ DNA Polymerase, AmpliTaq Gold™ DNA Polymerase, and the other components of the PCR mixture remain intact during UNG treatment, providing PCR amplification free of PCR product carryover. The dU-containing PCR product behaves like dT-containing DNA in blotting, cloning, sequencing, and most other post-PCR analyses.
For Research Use Only. Not for use in diagnostic procedures.
사양
PCR 방법qPCR, Standard PCR
중합효소DNA Polymerase
제품라인AmpErase™
제품 유형Uracil N-Glycosylase for PCR reactions
수량100 units
배송 조건Dry Ice
충분100 PCR Amplifications
용도(애플리케이션)Standard PCR
Unit Size100 units
구성 및 보관
Contains AmpErase™ Uracil N-glycosylase (UNG) (100 units). Sufficient for 100 PCR amplifications, 100 μl each. Store at -20°C.