pPICα A, B, and C vectors are 3.6 kb vectors used to express and secrete recombinant proteins in Pichia pastoris. Recombinant proteins are expressed as fusions to an N-terminal peptide encoding the Saccharomyces cerevisiae á-factor secretion signal. These vectors allow high-level, methanol inducible expression of the gene of interest in Pichia, and can be used in any Pichia strain including X-33, SMD1168H, and KM71H. pPICα vectors contain the following elements:
• Contains AOX1 promoter for tightly regulated, methanol-induced expression of the gene of interest • All three reading frames (A, B, C versions) are provided to facilitate in-frame cloning with the C-terminal peptide • α-factor secretion signal for directing secreted expression of the recombinant protein • Zeocin resistance gene for selection in both E. coli and Pichia • C-terminal peptide containing the c-myc epitope and a polyhistidine (6xHis) tag for detection and purification of a recombinant fusion protein
For Research Use Only. Not for use in diagnostic procedures.
사양
프로모터
AOX1
제품 유형
Pichia Expression Vector
항생제 내성 박테리아
Zeocin™ (ZeoR)
배송 조건
Room Temperature
단백질 태그
His Tag (6x), c-Myc Epitope Tag
클로닝 방법
Restriction Enzyme/MCS
벡터
pPIC
구성 및 보관
20 μg each of lyophilized pPICZ A, B, and C is supplied (Store at -20C)