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The pLenti6.2⁄V5-DEST™ Gateway™ Vector is a Gateway™-adapted ViraPower™ II lentiviral expression vector for lentiviral-based expression of a target gene in dividing and non-dividing mammalian cells. The vector has the CMV promoter for driving constitutive expression of the target gene and the PGK promoter for driving long-term, persistent expression of the Blasticidin stable selection marker.
Advantages
• Stable expression
• Long-term experiments
• High-throughput screening
• Accurate titer of functional virus (using Blasticidin method)
• Flexible and versatile Gateway™ recombination cloning technology
Key Features
• Human cytomegalovirus (CMV) immediate early promoter to control high-level expression of the gene of interest
• PKG Promoter for expression of Blasticidin selection marker
Kit includes
• pLenti6.2⁄V5-DEST™ Gateway™ Vector box
• One Shot™ Stbl3™ chemically competent E. coli (C7373-03)
Related SKUs
• 293FT Cell Line (R70007; R70007)
• ViraPower™ Lentiviral Support Kit (K4970-00)
• ViraPower™ Lentiviral Packaging Mix (K4975-00)
• Lipofectamine™ 2000 (11668-019; 11668-027)
For research use only. Not intended for any therapeutic or diagnostic use.
Advantages
• Stable expression
• Long-term experiments
• High-throughput screening
• Accurate titer of functional virus (using Blasticidin method)
• Flexible and versatile Gateway™ recombination cloning technology
Key Features
• Human cytomegalovirus (CMV) immediate early promoter to control high-level expression of the gene of interest
• PKG Promoter for expression of Blasticidin selection marker
Kit includes
• pLenti6.2⁄V5-DEST™ Gateway™ Vector box
• One Shot™ Stbl3™ chemically competent E. coli (C7373-03)
Related SKUs
• 293FT Cell Line (R70007; R70007)
• ViraPower™ Lentiviral Support Kit (K4970-00)
• ViraPower™ Lentiviral Packaging Mix (K4975-00)
• Lipofectamine™ 2000 (11668-019; 11668-027)
For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
사양
구성 또는 유도성 시스템
Constitutive
배달 유형
Lentiviral
프로모터
CMV
제품 유형
Lentiviral Expression Vector
선택 제제(진핵)
Blasticidin
선택 마커 프로모터
PGK Promoter
단백질 태그
V5 Epitope Tag
클로닝 방법
Gateway
벡터
pLenti
제품라인
Gateway™, ViraPower™
용도(애플리케이션)
Viral Expression
구성 및 보관
pLenti6.2⁄V5-DEST™ Gateway™ Vector Box contains pLenti6.2⁄V5-DEST, 40 μl of 150 ng⁄μl vector in 10 mM Tris-HCl, 1mM EDTA, pH 8.0 and pLenti6.2⁄V5-GW⁄lacZ control, 20 μl of 0.5 μg⁄μl vector in 10 mM Tris-HCl, 1mM EDTA, pH 8.0. Store at –20°C.
One Shot™ Stbl3™ chemically competent E. coli box contains transformation reagents including 20 single-use 50 μl aliquots of One Shot™ Competent Cells, S.O.C. medium, and pUC19 supercoiled plasmid control. Store at -80°C.
One Shot™ Stbl3™ chemically competent E. coli box contains transformation reagents including 20 single-use 50 μl aliquots of One Shot™ Competent Cells, S.O.C. medium, and pUC19 supercoiled plasmid control. Store at -80°C.
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