pOG44 Flp-Recombinase Expression Vector
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Invitrogen™

pOG44 Flp-Recombinase Expression Vector

In addition to the original pcDNA™5/FRT vector, four more Flp-In™ expression vectors-pcDNA5/FRT/V5-His-TOPO™, pSecTag/FRT/V5-His-TOPO™, pEF5/FRT/V5-DEST™, and pEF5/FRT/V5-D-TOPO™ (Figure 1)-are available for자세히 알아보기
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카탈로그 번호수량
V600520
V6005-20으로도 사용됨
20 μg
카탈로그 번호 V600520
V6005-20으로도 사용됨
제품 가격(KRW)
860,000
Online offer
Ends: 30-Sep-2025
1,011,000
할인액 151,000 (15%)
20 µg
-
카트에 추가하기
수량:
20 μg
제품 가격(KRW)
860,000
Online offer
Ends: 30-Sep-2025
1,011,000
할인액 151,000 (15%)
20 µg
카트에 추가하기
In addition to the original pcDNA™5/FRT vector, four more Flp-In™ expression vectors-pcDNA5/FRT/V5-His-TOPO™, pSecTag/FRT/V5-His-TOPO™, pEF5/FRT/V5-DEST™, and pEF5/FRT/V5-D-TOPO™ (Figure 1)-are available for cloning and expressing in the Flp-In™ System. These vectors offer a variety of cloning options and different promoter types. Vectors include the following features:

• Flp Recombinase Target (FRT) site for efficient integration into Flp-In™ Cell Lines
• Hygromycin resistance gene for convenient selection of integrants
• C-terminal V5 tag for easy detection of fusion proteins with the Anti-V5 Antibody
• 6xHis tag (pcDNA5/FRT/V5-His-TOPO™ and pSecTag/FRT/V5-His-TOPO™ vectors only) for rapid purification of fusion proteins on nickel-chelating resin

All vectors feature either the high-level CMV or EF-1α promoter. In addition pSecTag/FRT/V5-His-TOPO™ contains the Igκ leader sequence for secretion of recombinant protein.

Entry Options
pcDNA™5/FRT is suitable for restriction digest-mediated cloning. The other four Flp-In™ expression vectors offer two time-saving options for cloning into a Flp-In™ expression vector:
Five-minute TOPO™ Cloning

The pcDNA™5/FRT/V5-His and pSecTag/FRT/V5-His TOPO™ TA Expression Kits offer five-minute cloning of Taq-amplified PCR products directly into a Flp-In™ expression vector. The pEF5/FRT/V5 Directional TOPO™ Expression Kit allows five-minute directional cloning of PCR product generated with a proofreading enzyme. Using Directional TOPO™ Cloning, >90% of the resulting clones will be in the correct orientation for expression, reducing time spent colony screening.
Easy Recombination into a Gateway™ vector

The pEF5/FRT/V5-DEST™ vector is compatible with Gateway™ Technology* for easy transfer of your gene of interest into different vectors or host systems. The fast, efficient Gateway™ recombination reaction can be simultaneously performed with multiple destination vectors, saving you time when working with different systems.

Choice of Promoters
Flp-In™ expression vectors are available with the CMV or EF-1α promoter. The pEF5/FRT/V5-DEST™ and pEF5/FRT/V5-D-TOPO™ vectors contain the human EF-1É promoter for driving expression of the gene of interest. The EF-1α promoter expresses in a wide range of mammalian cell types, including those where the CMV promoter expression is absent or inconsistent. This promoter may be more appropriate for long-term gene expression in some cell types and is recommended for expression in the BHK and 3T3 pre-made Flp-In™ Cell Lines. The pcDNA5/FRT/V5-His-TOPO™ and pSecTag/FRT/V5-His-TOPO™ vectors carry the CMV promoter for high-level constitutive expression of your gene of interest in most cell types.
For Research Use Only. Not for use in diagnostic procedures.
사양
구성 또는 유도성 시스템Constitutive
배달 유형Transfection
용도(애플리케이션)Targeted Integration
제품 유형Flp-In System Expression Vector
수량20 μg
선택 제제(진핵)None
벡터pOG44
클로닝 방법TOPO, Gateway
제품라인Flp-In™
프로모터CMV
단백질 태그Untagged
Unit Size20 µg
구성 및 보관
The pcDNA™5/FRT/V5-His TOPO™ TA Expression Kit, pSecTag/FRT/V5-His TOPO™ TA Expression Kit, and the pEF5/FRT/V5 Directional TOPO™ Expression Kit contain two boxes. The TOPO™ Cloning box contains all of the reagents necessary to clone a Taq-amplified PCR product (TOPO™ TA) or a PCR product generated with a proofreading enzyme (Directional TOPO™), including 200 ng of topoisomerase Iactivated vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, primers for sequencing, and an expression control plasmid. Store at -20°C. The One Shot™ Competent Cell box contains all of the reagents necessary for transformation, including twenty-one 50-μl aliquots of One Shot™ TOP10 Chemically Competent E. coli, S.O.C. medium, and pUC19 supercoiled control plasmid. Store at -80°C. The pEF5/FRT/V5-DEST™ vector contains 6 μg of lyophilized vector and a control plasmid. Store at -20°C once resuspended. All reagents are guaranteed stable for 6 months when properly stored.

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