This pcDNA™3.1(+) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin™ selectable marker and a forward-orientation multiple cloning site.
The pcDNA™3.1 Expression Vector Family Three untagged versions of pcDNA™3.1 (available separately), each with a different selectable marker (Geneticin™, Zeocin™, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features:
• Cytomegalovirus (CMV) enhancer-promoter for high-level expression • Large multiple cloning site in either forward (+) or reverse (-) orientations • Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence for enhanced mRNA stability • SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (i.e., COS-1 and COS-7) • Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli
For Research Use Only. Not for use in diagnostic procedures.
사양
배달 유형
Transfection
프로모터
CMV
제품 유형
Mammalian Expression Vector
선택 제제(진핵)
Geneticin™ (G-418)
단백질 태그
Untagged
클로닝 방법
Restriction Enzyme/MCS
벡터
pcDNA
제품라인
pcDNA™
용도(애플리케이션)
Constitutive Expression
구성 및 보관
20 μg of this pcDNA™3.1(+) vector, as well as an expression control, are supplied supercoiled and lyophilized. Store at -20°C. Vectors are guaranteed stable for 6 months when properly stored.