The pcDNA™4/HisMax A, B, & C vectors, 20 μg each, are supercoiled and provided lyophilized. 20 μg of pcDNA™4/HisMax/lacZ control plasmid is also provided. Store vectors at -20°C. The pcDNA™4/HisMax TOPO™TA Expression Kit contains two boxes. The TOPO™ TA box contains 200 ng of linearized and topoisomerase I-activated pcDNA4/HisMax-TOPO™ vector, dNTPs, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a lacZ expression control plasmid.
Store this box at -20°C. The One Shot™ box contains transformation reagents including twenty-one 50-μl aliquots of TOP10 E. coli, S.O.C. medium, and a pUC19 supercoiled control plasmid. The One Shot™ box should be stored at -80°C. Guaranteed stable for 6 months when properly stored.