pSecTag2 and pSecTag2/Hygro are mammalian expression vectors designed for the secretion, purification, and detection of fusion proteins. Each vector has a large multiple cloning site in three reading frames to simplify cloning in frame with the N-terminal secretion signal. The vectors (Figure 1) offer the following features:
• Secretion signal from the V-J2-C region of the mouse Ig kappa-chain for efficient secretion of recombinant proteins (Figure 2) • Cytomegalovirus (CMV) promoter for high-level constitutive expression • C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an Anti-His(C-term) Antibody • C-terminal c-myc epitope for detection with an Anti-myc Antibody • Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence to enhance mRNA stability • SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (e.g., COS-1, COS-7)
The pSecTag2 vectors carry the Zeocin™ resistance gene for cost-effective selection in mammalian cells. Zeocin™ selection can also be used in E. coli.
The pSecTag2/Hygro vectors have the Hygromycin B resistance gene for selection of stable mammalian cell lines.
For Research Use Only. Not for use in diagnostic procedures.
사양
배달 유형
Transfection
프로모터
CMV
제품 유형
Mammalian Expression Vector
선택 제제(진핵)
Zeocin™
단백질 태그
His Tag (6x), c-Myc Epitope Tag, IgK Leader Sequence
클로닝 방법
Restriction Enzyme/MCS
벡터
pSec
용도(애플리케이션)
Secreted Expression
구성 및 보관
20 μg of each vector and a PSA expression control are supplied supercoiled and lyophilized. Store at -20°C. Vectors are guaranteed stable for 6 months when properly stored.