EZ-Link™ Plus Activated Peroxidase
EZ-Link™ Plus Activated Peroxidase
Thermo Scientific™

EZ-Link™ Plus Activated Peroxidase

Thermo Scientific Pierce Plus Activated Peroxidase is an amine-reactive form of horseradish peroxidase (HRP) that provides coupling efficiencies of greaterRead more
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Catalog NumberQuantity
314871 mg
314885 x 1mg
Catalog number 31487
Price (TWD)/ Each
Price:5,950.00
Online offer:4,100.00Web orders only. Excludes Supply Centers.
(ends 01-Jan-2025)
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Quantity:
1 mg
Request bulk or custom format
Price (TWD)/ Each
Online offer:4,100.00Web orders only. Excludes Supply Centers.
(ends 01-Jan-2025)
Add to cart
EZ-Link™ Plus Activated Peroxidase
Catalog number31487
Price (TWD)/ Each
Online offer:4,100.00Web orders only. Excludes Supply Centers.
(ends 01-Jan-2025)
-
Add to cart
Thermo Scientific Pierce Plus Activated Peroxidase is an amine-reactive form of horseradish peroxidase (HRP) that provides coupling efficiencies of greater than 95% with antibodies and other proteins. This product consists of 5 mg of lyophilized HRP and is sufficient to modify 5 mg of Immunoglobulin (IgG)

Features of Thermo Scientific Pierce Plus Activated Peroxidase:

Activated HRP – periodate-treated, aldehyde-activated horseradish peroxidase, ready for conjugation to antibodies and other proteins at sites of primary amines (e.g., lysines)
Permanent conjugation – reacts efficiently (95%) with primary amines to form covalent amide bonds upon treatment with sodium cyanoborohydride (included in kit)
High activity HRP – enzyme activity is 120 to 200 units/mg; lyophilized, activated enzyme is stable for at least 12 months at -20°C
Convenient quantities – each 1 mg-quantity of activated enzyme is sufficient for reaction with 1 mg of IgG to produce about 0.5 mL of conjugate
Customizable – vary the molar ratios, reaction buffer and pH, and other parameters to acheive conjugates with different levels of HRP incorporation and activity

Plus Activated Peroxidase is horseradish peroxidase (HRP) enzyme, whose native carbohydrates (sugars) have been gently oxidized with periodate to produce amine-reactive aldehyde groups. These carbonyls of Plus Activated Peroxidase spontaneously and efficiently crosslink with primary amines on antibody or other proteins. The method is more effective than other amine-reactive chemistries, such as glutaraldehyde coupling, which often causes polymerization and a greater degree of conjugate inactivation. The pre-activated enzyme eliminates the difficulties inherent in preparing and validating activated peroxidase from scratch. The kit provides the enzyme, accessory reagents and protocol to easily produce high-performance conjugates for Western blotting, ELISA and other detection techniques.

Related Products
EZ-Link™ Plus Activated Peroxidase Kit
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical ReactivityAmine
Quantity1 mg
Reactive MoietyAldehyde
SolubilityWater
FormSolid
Product LineEZ-Link™
Label TypeEnzyme Labeled
Label or DyeHRP (Horseradish Peroxidase)
Unit SizeEach
Contents & Storage
Note: EZ-Link Plus Activated Peroxidase contains 1-3 moles of aldehyde per mole of peroxidase.

Storage: Upon receipt store product protected from moisture at -20°C. Product is shipped with an ice pack.