Gibco CTS Viral Production Medium is a chemically defined, serum-free, protein-free, animal-origin-free, and phenol-red-free medium that is part of the CTS AAV-MAX Helper-Free AAV Production System. CTS Viral Production Medium is specifically developed for growth and transfection of CTS Viral Production Cells 2.0 (suspension-adapted HEK293F-derived cells) for adeno-associated virus (AAV) production.

Features of CTS Viral Production Medium include:

• Supports growth and transfection of CTS Viral Production Cells 2.0 from shake flask to bioreactor scales
• Enables production of high AAV titers, greater than 5 x 10¹⁰ vg/mL (unpurified)
• Formulated with GlutaMAX Supplement, so does not require further supplementation. With the exception of containing GlutaMAX Supplement, the formulation of this product is identical to research-grade Viral Production Medium.
• Manufactured in conformity with cGMP for medical devices (21 CFR Part 820) and follows USP <1043> and European Pharmacopoeia (Ph. Eur.) 5.2.12 recommendations. Drug Master File and Regulatory Support File available upon request

The CTS AAV-MAX Helper-Free AAV Production System is a scalable, high-titer platform for suspension AAV production with research-grade and GMP versions to enable seamless transition from research to commercial manufacturing. Other components of the CTS AAV-MAX system include CTS Viral Production Cells 2.0, CTS AAV-MAX Transfection Kit, CTS Viral-Plex Complexation Buffer, and Viral Production Medium, AGT.