Workflow-driven experiment creation, method processing, and result review
Intact protein workflow:

• Rapidly measure the intact molecular weight of biotherapeutics, including proteins and oligonucleotides, for structural confirmation and characterization
• Confidently determine deconvoluted molecular weights
• Target protein sequence matching to identify n-linked glycosylations and other common modifications
• Identify Antibody Drug Conjugates (ADC) using sliding window algorithm
• Batch-to-batch comparison

Peptide mapping workflow:

• Confirm amino acid sequence with a novel MS² prediction algorithm, providing extra confidence for sequence assignments
• Identify the site and type of expected and unknown PTMs
• Map disulfide bonds
• Detect low-level impurities and sequence variants
• Perform error-tolerant searches for unexpected modifications
• Create de novo sequence searches

Multi Attribute Methods (MAM):

• MS-based MAM for characterization and relative quantification of PTMs
• Features streamlined processes ranging from discovery peptide mapping to critical quality attributes (CQAs) monitoring in GMP environments

Top down workflow:

• Features a simple workflow for sequencing intact protein molecules using ProSightBP as the core algorithm
• Includes multiple raw file comparison with combined interactive fragmentation coverage maps
• Supports multiple modes of fragmentation CID, HCD, ETD, EThcD and UVPD

Oligonucleotide workflow:

• Support for oligonucleotide sequences with built in customized building block subunits, including base, backbone linker, 2' ribose, 3' terminal and 5' terminal
• Automated annotation and confirmation of oligonucleotide sequence with a novel MS² prediction algorithm, providing extra confidence in oligonucleotide sequencing