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Cell Permeability | Cell-permeant |
For Use With (Application) | Cell Tracing, Cell Tracker |
For Use With (Equipment) | Fluorescence Microscope |
Product Type | Calcein Blue, AM |
Dye Type | Other Label(s) or Dye(s) |
Emission | 449 nm |
Quantity | 1 mg |
Reagent Type | Cell Tracker Compounds, Cell Labeling Reagents |
Shipping Condition | Room Temperature |
Target Enzyme | Esterase |
Excitation/Emission | 322/437 nm |
Molecular Weight (g/mol) | 465.41 |
Unit Size | Each |
Cell Permeability | Cell-permeant |
For Use With (Application) | Cell Tracing, Cell Tracker |
For Use With (Equipment) | Fluorescence Microscope |
Product Type | Calcein, AM |
Dye Type | Other Label(s) or Dye(s) |
Emission | 517 nm |
Quantity | 20 x 50 μg |
Reagent Type | Cell Tracker Compounds, Cell Labeling Reagents |
Shipping Condition | Room Temperature |
Target Enzyme | Esterase |
Molecular Weight (g/mol) | 994.86 |
Unit Size | Each |
Cell Permeability | Cell-permeant |
For Use With (Application) | Cell Tracing, Cell Tracker |
For Use With (Equipment) | Fluorescence Microscope |
Product Type | Calcein, AM |
Dye Type | Other Label(s) or Dye(s) |
Emission | 517 nm |
Excitation Wavelength Range | 494 nm |
Quantity | 1 mg |
Reagent Type | Cell Tracker Compounds, Cell Labeling Reagents |
Shipping Condition | Room Temperature |
Target Enzyme | Esterase |
Unit Size | Each |
Cell Permeability | Cell-permeant |
Concentration | 1 mg/mL |
For Use With (Application) | Cell Tracing, Cell Tracker |
For Use With (Equipment) | Fluorescence Microscope |
Product Type | Calcein, AM (1 mg/mL solution in dry DMSO) |
Dye Type | Other Label(s) or Dye(s) |
Emission | 517 nm |
Excitation Wavelength Range | 494 nm |
Quantity | 1 mL |
Reagent Type | Cell Tracker Compounds, Cell Labeling Reagents |
Shipping Condition | Room Temperature |
Target Enzyme | Esterase |
Unit Size | Each |
Cell Permeability | Cell-permeant |
For Use With (Application) | Cell Tracing, Cell Tracker |
For Use With (Equipment) | Fluorescence Microscope |
Product Type | Calcein Green, AM |
Dye Type | Other Label(s) or Dye(s) |
Emission | 495 nm |
Excitation Wavelength Range | 515 nm |
Format | Special packaging |
Product Line | CellTrace™ |
Quantity | 20 x 50 μg |
Reagent Type | Cell Tracker Compounds, Cell Labeling Reagents |
Shipping Condition | Room Temperature |
Target Enzyme | Esterase |
Unit Size | Each |
Cell Permeability | Cell-impermeant |
For Use With (Application) | Cell Tracing, Cell Tracker |
For Use With (Equipment) | Fluorescence Microscope |
Product Type | Calcein, high purity |
Dye Type | Other Label(s) or Dye(s) |
Emission | 517 nm |
Excitation Wavelength Range | 494 nm |
Quantity | 100 mg |
Reagent Type | Cell Tracker Compounds, Cell Labeling Reagents |
Shipping Condition | Room Temperature |
Unit Size | 100 mg |
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C1429 | Each | Calcein Blue, AM | 1 mg | Price: 8,210.00 Online offer: 5,747.00 (ends 01-Oct-2024) Your price: | - | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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C3100MP | Each | Calcein, AM | 20 x 50 μg | Price: 18,800.00 Online offer: 13,943.00 (ends 01-Oct-2024) Your price: | - | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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C1430 | Each | Calcein, AM | 1 mg | *** | *** | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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C3099 | Each | Calcein, AM (1 mg/mL solution in dry DMSO) | 1 mL | Price: 17,000.00 Online offer: 13,466.00 (ends 01-Oct-2024) Your price: | - | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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C34852 | Each | Calcein Green, AM | 20 x 50 μg | *** | *** | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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C481 | 100 mg | Calcein, high purity | 100 mg | Price: 9,610.00 Online offer: 6,727.00 (ends 01-Oct-2024) Your price: | - | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Cell vitality as measured by intracellular esterase activity is a recognized parameter of cell health. Live cells are distinguished by the presence of ubiquitous intracellular esterase activity, determined by the enzymatic conversion of the virtually non-fluorescent cell permeant Calcein AM to the intensely fluorescent Calcein Green (ex/em 494/517 nm) or Calcein Blue (ex/em 360/449 nm). Apoptotic and dead cells with compromised membranes do not retain Calcein.
The acetoxymethyl (AM) ester derivatives of fluorescent indicators make up one of the most useful groups of compounds for the study of live cells. Once inside the cell, the lipophilic blocking groups are cleaved by nonspecific esterases, resulting in a charged form that is retained in cells to a much greater extent than its parent compound.
In addition to cell viability assays, Calcein AM is also used to study cell migration, cell adhesion, and cellular uptake processes. Its fluorescence properties make it a versatile tool for various applications in cell biology research.
Application of the non-AM Calcein cell-impermeant dye:
Calcein, AM requires esterase cleavage of the acetoxymethyl (AM) ester to become fluorescent. Liposomes don't have esterases unless specifically constructed to include the enzyme. The water-soluble, non-AM form of Calcein (Cat. No. C481), does not require esterase cleavage to be fluorescent.
This versatile green fluorophore is cell impermeant and is commonly used for cell tracing and in studies of endocytosis and gap junctions.
Features of Calcein AM cell-permeant dye:
Application of the Calcein AM cell-permeant dye:
Calcein AM can be used in various applications, including cell viability assays, cell migration and invasion studies, intracellular calcium imaging, cell labeling and tracing, and fluorescence microscopy and flow cytometry.