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Thermo Scientific TheraPure phi29 DNA Polymerase is a highly processive DNA polymerase with strand displacement activity. This capability allows for highly efficient isothermal DNA amplification using a technique referred to as rolling circle amplification (RCA). This enzyme is produced recombinantly in E. coli cells using a cloned gene from Bacillus subtilis phage phi29. phi29 DNA Polymerase also possesses 3'→5' proofreading exonuclease activity that acts preferentially on single-stranded DNA or RNA.
The enzyme was developed specifically for preparation of DNA template used in the in vitro transcription reaction to produce nucleic acid therapeutics such as mRNA.
Designed for therapeutics manufacturing process development
TheraPure phi29 DNA Polymerase helps accelerate RNA or DNA vaccine and therapeutics development by providing:
Comprehensive quality system
TheraPure phi29 DNA Polymerase is supported by an extensive quality system and documentation.
Enzyme activity
One unit of the enzyme incorporates 0.5 pmol of dCMP into a polynucleotide in 10 min. at 30°C.
*TheraPure GMP refers to the quality level of the raw, ancillary, or starting materials to be used for further manufacturing. TheraPure GMP products are manufactured in facilities with ISO 9001–certified quality management systems that operate in accordance with relevant good manufacturing practice (GMP) principles, as outlined in ICH Q7 or equivalent guidance documents or standards.