FluoZin™-3, AM, cell permeant

The FluoZin™-3 indicator is a Zn²⁺-selective indicator with a structure that resembles fluo-4. FluoZin™-3 exhibits high Zn²⁺-binding affinity that is unperturbed by Ca²⁺ concentrations up to at least 1 μM. In addition, FluoZin™-3 exhibits a >50-fold increase in fluorescence in response to saturating levels of Zn²⁺. Although imaging applications of FluoZin™-3 are predominant, cell-based microplate assays and flow cytometry protocols have also been developed. FluoZin™-3 indicator is available in a cell-permeant and a cell-impermeant form.

Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›

Fluorescent Zinc Indicators Specifications:
• Label (Ex/Em): FluoZin™-3 (∼494/516 nm)
• K_d (Zn²⁺) (in buffer): ∼15 nM
• Lyophilized product may be dissolved in DMSO for use
• Product is typically loaded into cells by adding the dissolved indicator to medium containing cells

Find Fluorescent Indicators for Zinc and Other Metal Ions
We offer a number of fluorescent indicators to determine polyvalent cation concentrations inside cells, to follow metal ion transport through ion channels, to make measurements in environmental samples, and more. Review Fluorescent Indicators for Zn²⁺ and Other Metal Ions—Section 19.7 in the Molecular Probes™ Handbook for more information on these products.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.