Tango™ GPR119-bla U2OS Cells

Name: Tango™ GPR119- bla U2OS Cells
SKU: K1777

The Tango™ GPR119-bla U2OS cells contain the human G-protein Coupled Receptor 119 (GPR119) linked to a TEV protease site and Read more

Catalog number K1777

Price (TWD)/ 8 x 10⁶ cells

The Tango™ GPR119-bla U2OS cells contain the human G-protein Coupled Receptor 119 (GPR119) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ GPR119-bla U2OS cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ GPR119-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession NumberNM_178471.1

Assay EntryCell-based beta-arrestin recruitment

Cell StateDividing Cells

Cytoplasmic TailNative Tail

Detection MethodFluorescence

Gene SymbolGPR119

Population StatusSingle Cell Clone Origin

Product LineGeneBLAzer™

Protein FamilyGPCR (G-Protein Coupled Receptors)

Quantity8 x 10⁶ Cells

ReadoutEnd Point

Reporter GeneBLA (Beta-Lactamase)

Target EntryGPR119

Cell LineU2OS

System TypeTango™

Druggable TargetGPCR

Unit Size8 x 10⁶ cells

Contents & Storage

LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.

Includes:
• Tango™ GPR119-bla U2OS cells
• Tango™ GPR119-bla U2OS Assay Protocol
• Certificate of Analysis

The Tango™ GPR119-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.