Search Thermo Fisher Scientific
Invitrogen™
Terminal Deoxynucleotidyl Transferase, recombinant
Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT) is a DNA polymerase that catalyzes the addition of deoxynucleotides to the 3fi hydroxyl terminus 深入閱讀
Have Questions?
變更視圖
| 產品號碼 | Quantity |
|---|---|
| 10533065 | 500 U |
| 10533073 | 3 x 500 U |
產品號碼 10533065
價格 (TWD)/ 500 units
價格11,000.00
線上優惠7,700.00僅限網絡訂單。不包括供應中心
(ends 01-Jan-2025)
您的價格登入登入以查看您的帳戶報價。需要一個帳戶嗎？今天就向我們註冊。
-
Quantity:
500 U
價格 (TWD)/ 500 units
線上優惠7,700.00僅限網絡訂單。不包括供應中心
(ends 01-Jan-2025)
Terminal Deoxynucleotidyl Transferase, recombinant
產品號碼10533065
價格 (TWD)/ 500 units
線上優惠7,700.00僅限網絡訂單。不包括供應中心
(ends 01-Jan-2025)
-
Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT) is a DNA polymerase that catalyzes the addition of deoxynucleotides to the 3fi hydroxyl terminus of DNA. A TdT Technical Bulletin is available.
Applications: Homopolymer tailing of vector and insert for cloning. Labeling oligonucleotides with biotin (1,2), 32P- or 35S-label (3), or in apoptosis (TUNEL) (4,5).
Source: Purified from E. coli clone of calf thymus TdT.
Performance and Quality Testing: Endonuclease, 3´ and 5´ exodeoxyribonuclease, and levels of incorporation tested.
Unit Definition: One unit incorporates 1 nmol dATP into acid-precipitable material in 1 h at 37°C, using d(pA)50 as a primer.
Hazard Warning: Toxic; potassium cacodylate contained in reaction buffer. Also contains cobalt chloride, a highly toxic chemical.
See MSDS.
Unit Reaction Conditions: 0.2 M potassium cacodylate (pH 7.2), 10 mM MgO4 C4 H6 , 1 mM 2-mercaptoethanol, 0.5 mg/ml BSA,
100 flM d(pA)50, 1 mM [3H]dATP, and enzyme in 0.15 ml for 1 h at 37°C.
Applications: Homopolymer tailing of vector and insert for cloning. Labeling oligonucleotides with biotin (1,2), 32P- or 35S-label (3), or in apoptosis (TUNEL) (4,5).
Source: Purified from E. coli clone of calf thymus TdT.
Performance and Quality Testing: Endonuclease, 3´ and 5´ exodeoxyribonuclease, and levels of incorporation tested.
Unit Definition: One unit incorporates 1 nmol dATP into acid-precipitable material in 1 h at 37°C, using d(pA)50 as a primer.
Hazard Warning: Toxic; potassium cacodylate contained in reaction buffer. Also contains cobalt chloride, a highly toxic chemical.
See MSDS.
Unit Reaction Conditions: 0.2 M potassium cacodylate (pH 7.2), 10 mM MgO4 C4 H6 , 1 mM 2-mercaptoethanol, 0.5 mg/ml BSA,
100 flM d(pA)50, 1 mM [3H]dATP, and enzyme in 0.15 ml for 1 h at 37°C.
For Research Use Only. Not for use in diagnostic procedures.
規格
Shipping ConditionApproved for shipment on Wet or Dry Ice
EnzymeTerminal Deoxynucleotidyl Transferase
Compatible Buffer5X Buffer, Reaction Buffer
Quantity500 U
Product TypeTdT
Unit Size500 units
內容物與存放
Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT) is supplied with vial of 5X buffer [500 mM potassium cacodylate (pH 7.2), 10 mM CoCl2 , 1 mM DTT]. Store at -20°C.