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引用資料與參考文獻 (3)
Invitrogen™
1 Kb Plus DNA Ladder
Invitrogen 1 Kb Plus DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of深入閱讀
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| 產品號碼 | Quantity |
|---|---|
| 10787018 | 500 Applications |
| 10787026 | 2000 Applications |
產品號碼 10787018
價格 (TWD)
***
Quantity:
500 Applications
價格 (TWD)
***
Invitrogen 1 Kb Plus DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of 100 bp to 15,000 bp. 1 Kb Plus DNA Ladder consists of 18 individual chromatography-purified DNA fragments and has a reference band at 1,500 bp for easy orientation.
1 Kb DNA Ladder is ideal for separation on 0.8–1% agarose gels.
Highlights of 1 Kb Plus DNA Ladder:
• Sharp, clear bands—chromatography purified fragments for consistent and reliable results
• Convenient—provided with 10X BlueJuice Gel Loading Buffer for tracking of sample DNA migration
• Precise—an exact amount of DNA in each band
Product use
The double-stranded DNA ladder can be visualized on 0.8–1% agarose gels after ethidium bromide or SYBR Safe staining. The ladder is designed with a uniform intensity of DNA bands for a clear view of each band. An exact amount of DNA in each band allows approximate quantification of DNA samples.
This ladder can be radiolabeled with T4 polynucleotide kinase or T4 DNA polymerase.
1 Kb DNA Ladder is ideal for separation on 0.8–1% agarose gels.
Highlights of 1 Kb Plus DNA Ladder:
• Sharp, clear bands—chromatography purified fragments for consistent and reliable results
• Convenient—provided with 10X BlueJuice Gel Loading Buffer for tracking of sample DNA migration
• Precise—an exact amount of DNA in each band
Product use
The double-stranded DNA ladder can be visualized on 0.8–1% agarose gels after ethidium bromide or SYBR Safe staining. The ladder is designed with a uniform intensity of DNA bands for a clear view of each band. An exact amount of DNA in each band allows approximate quantification of DNA samples.
This ladder can be radiolabeled with T4 polynucleotide kinase or T4 DNA polymerase.
For Research Use Only. Not for use in diagnostic procedures.
規格
Concentration0.5 μg/μL
Gel CompatibilityAgarose gel
Green FeaturesSustainable packaging
Kit Contents1 μL DNA Ladder (500 ng), 1 μL Sample Loading Buffer, 500 Applications
No. of Reactions500 Applications
Product TypeDNA Ladder
Quantity500 Applications
Ready to LoadNo
Sample Loading Volume1 mL
Shipping ConditionApproved for shipment at Room Temperature or on Dry Ice
TechnologyIndividual chromatography-purified DNA fragments
Volume (Metric)500 μL
Gel TypeAgarose
Size Range100 to 15000 bp
Unit SizeEach
內容物與存放
• 500 µL 1 Kb Plus DNA Ladder
• 1 mL 10X BlueJuice Gel Loading Buffer
Store at -20°C.
• 1 mL 10X BlueJuice Gel Loading Buffer
Store at -20°C.
圖表
1 Kb Plus DNA Ladder
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批號Certificate TypeDateCatalog Number(s)
3274295Certificate of AnalysisJul 04, 202510787026
3274321Certificate of AnalysisJul 04, 202510787026
3251705Certificate of AnalysisJun 02, 202510787026
3248404Certificate of AnalysisMay 28, 202510787018
3247828Certificate of AnalysisMay 28, 202510787018
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安全資料表
SDS常見問答集 (常見問題)
Sequences of Invitrogen DNA and RNA ladders are proprietary.
Invitrogen DNA ladders contain linear dsDNA fragments.
Invitrogen DNA ladders are composed of double-stranded DNA fragments only.
Here are a few reasons why you might see smearing of the bands:
- The DNA was degraded. Avoid nuclease contamination of DNA standards.
- Too much DNA was loaded on the gel. Decrease the amount of DNA in the gel.
- The DNA was contaminated by protein. Remove proteins by phenol extraction before electrophoresis.
- For small DNA, the bands may have diffused during staining. Add the ethidium bromide before electrophoresis.
- For radiolabeled DNA, labeling was performed by nick translation. Label the DNA by replacement synthesis with T4 DNA polymerase or label the 5' end with T4 polynucleotide kinase.
- Improper electrophoresis conditions were used. Do not allow voltage to exceed ~20 V/cm. Maintain a temperature <30°C during electrophoresis. Check that the electrophoresis buffer used has sufficient buffering capacity.
- The DNA contained too much salt. Remove excess salt by ethanol precipitation before electrophoresis.
This can happen if the marker was heated. Please ensure that the ladders are not heated before use.
引用資料與參考文獻 (3)
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引用資料與參考文獻
Abstract
Efficient intracellular assembly of papillomaviral vectors.
Journal:J Virol
PubMed ID:14694107
Although the papillomavirus structural proteins, L1 and L2, can spontaneously coassemble to form virus-like particles, currently available methods for production of L1/L2 particles capable of transducing reporter plasmids into mammalian cells are technically demanding and relatively low-yield. In this report, we describe a simple 293 cell transfection method for efficient
Activation of protein kinase C beta II by the stereo-specific phosphatidylserine receptor is required for phagocytosis of apoptotic thymocytes by resident murine tissue macrophages.
Journal:J Biol Chem
PubMed ID:12114511
We showed previously that protein kinase C (PKC) is required for phagocytosis of apoptotic leukocytes by murine alveolar (AMø) and peritoneal macrophages (PMø) and that such phagocytosis is markedly lower in AMø compared with PMø. In this study, we examined the roles of individual PKC isoforms in phagocytosis of apoptotic
Bisindolylmaleimide VIII enhances DR5-mediated apoptosis through the MKK4/JNK/p38 kinase and the mitochondrial pathways.
Journal:J Biol Chem
PubMed ID:12034736
Bisindolylmaleimide VIII (Bis VIII) has been previously shown to enhance Fas-mediated apoptosis through a protein kinase C-independent mechanism. In the present study, we examined the effect of Bis VIII on apoptosis induced by DR5 (TRAIL-R2), using an agonistic anti-human DR5 monoclonal antibody, TRA-8. Our results demonstrated that Bis VIII was
3 total citations