Use this kit to isolate pure and viable untouched T-cells from PBMC by negative isolation. The kit depletes B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human T-cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free T-cells are truly untouched, and in perfect shape for any functional assay and application.
• High purity, recovery and viability of human untouched T cells • Easy to use and to scale up • No columns required
Methodology: An antibody mix towards the non-T cells is added to the sample and allowed to bind to the cells. Dynabeads™ are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human T-cells can be directly analyzed in a flow cytometer and used in any application.
Note: A negative isolation strategy isolates pure and truly untouched cells. When maximum cell purity is critical, a positive cell isolation strategy is recommended
Starting Sample: PBMC
For Research Use Only. Not for use in diagnostic procedures.
規格
Cell Type
T Cells (Whole Population)
Product Type
Cell Isolation Kit
Final Product Type
Cells
Isolation Technology
Negative Isolation
No. of Cells
Processes ∼1 x 109 cells total
No. of Tests
100 Isolations
Output Viability
>95%
Product Line
DYNAL™, Dynabeads™, Untouched™
Quantity
1 kit
Sample Type
PBMC
Shipping Condition
Room Temperature
Starting Material Cell No.
1 x 107 PBMCs per isolation
Target Species
Human
內容物與存放
The Kit Contains: 10 mL Depletion Dynabeads™ and 2 mL Antibody Mix with monoclonal antibodies towards human CD14, CD16 (a and b), CD19, CD36, CD56, CD123 and CD235a (Glycophorin A).