Hybridoma-SFM is a serum-free, very low-protein medium specifically developed to support the growth of hybridomas for antibody production. Hybridoma-SFM features:

• Ability to support suspension and stationary hybridomas and myelomas
• Complete, ready-to-use formulation
• Serum-free, very low-protein formulation

Ability to support suspension and stationary hybridomas and myelomas
Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Hybridoma-SFM provides high cell densities and IgG production. Cholesterol-dependent cultures such as NS0 require the addition of 250X Cholesterol Lipid Concentrate.

Complete, ready-to-use formulation
Hybridoma-SFM is a complete medium that is ready to use. No supplementation is required.

Serum-free, very low-protein formulation
Hybridoma-SFM is a serum-free, very low-protein formulation, allowing for easier purification of your protein of interest. Hybridoma-SFM contains <20 μg/mL total protein.

Product use
Customers using Gibco™ Hybridoma-SFM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP manufacturing and quality system
Hybridoma-SFM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.