AccuPrime™ Pfx DNA Polymerase

Invitrogen™

AccuPrime™ Pfx DNA Polymerase

Name: AccuPrime™ Pfx DNA Polymerase
SKU: 12344024

AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis.

變更視圖

產品號碼	No. of Reactions
12344024	200 Reactions
12344032	1000 Reactions

2 選項

產品號碼 12344024

價格 (TWD)

***

新增至購物車

No. of Reactions:

200 Reactions

Request bulk or custom format

價格 (TWD)

***

新增至購物車

AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3'→5' exonuclease) activity. Platinum anti-Pfx DNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity (see figure). Thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over Platinum Pfx alone.

Benefits of AccuPrime Pfx DNA Polymerase

Greater than 26 times higher fidelity than Taq DNA polymerase
Amplification of fragments up to 12 kb
Minimized PCR optimization

Applications

Amplification of DNA from complex genomic, viral, and plasmid templates
RT-PCR
High-fidelity PCR
Hot-start PCR

Notes

AccuPrime Pfx DNA Polymerase is especially effective where very high levels of fidelity are required.
The 10X AccuPrime Pfx reaction mix contains MgSO₄, dNTPs, and thermostable proteins that enhance specific primer-template hybridization during each PCR cycle.

For Research Use Only. Not for use in diagnostic procedures.

規格

Fidelity (vs. Taq)26X

Hot StartBuilt-In Hot Start

No. of Reactions200 Reactions

OverhangBlunt

PolymeraseAccuPrime Pfx DNA Polymerase

Quantity200 Reactions

Reaction FormatSeparate Components

Shipping ConditionWet or Dry Ice

Size (Final Product)12 kb or less

Volume100 μL

Detection MethodPrimer-probe

For Use With (Application)Hot-start PCR, High-fidelity PCR

GC-Rich PCR PerformanceLow

Reaction SpeedStandard

Unit SizeEach

內容物與存放

• AccuPrime Pfx DNA Polymerase (1 x 100 μL at 2.5 U/μL)
• 10X AccuPrime Pfx Reaction Mix (1 x 1 mL)
• 50 mM MgSO₄ (1 x 1 mL)

Store at -20°C.

常見問答集 (常見問題)

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

There is a green color in my lyophilized oligo. Can I still use it?

If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

View all AccuPrime™ Pfx DNA Polymerase FAQs

文件與下載

證書

依批號或部分批號搜尋

搜尋

要求 COA