Lumio™ Technology is designed for simple fluorescent detection of recombinant protein. The Lumio™ tag is a small (six amino acid) sequence that binds a fluorescent substrate, allowing you to visually detect protein localization in mammalian cells. The Mammalian Lumio™ Gateway™ vectors (Figure 1) offer the following features:

• Lumio™ tag for accurate in vitro and in vivo protein detection
• CMV promoter for high-level constitutive expression
• tR sites for efficient recombination with Gateway™ entry clones
• Blasticidin resistance gene for fast, efficient selection

Use Mammalian Lumio™ Gateway™ vectors for reliable and consistent protein expression, detection, and localization in mammalian cells (Figure 2). Vectors are available for generating protein with an N- and C-terminal Lumio™ fusion tag. In addition, each Mammalian Lumio™ Gateway™ Kit includes a Lumio™ Green, Red, or Dual In-Cell Detection Kit. The Dual In-Cell Detection Kit is ideal for doing two color “pulse-chase” experiments to examine protein localization over time.