DNA Polymerase I, Large (Klenow) Fragment is a DNA polymerase enzyme that lacks the 5' to 3' exonuclease activity of深入閱讀
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產品號碼
Quantity
18012039
500 Units
18012021
100 Units
18012096
亦稱為 18012-096
2 x 500 unit
產品號碼 18012039
價格 (TWD)/ 500 units
價格27,700.00
線上優惠19,390.00僅限網絡訂單。不包括供應中心
(ends 01-Oct-2024)
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Quantity:
500 Units
價格 (TWD)/ 500 units
線上優惠19,390.00僅限網絡訂單。不包括供應中心
(ends 01-Oct-2024)
新增至購物車
DNA Polymerase I, Large (Klenow) Fragment
產品號碼18012039
價格 (TWD)/ 500 units
線上優惠19,390.00僅限網絡訂單。不包括供應中心
(ends 01-Oct-2024)
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新增至購物車
DNA Polymerase I, Large (Klenow) Fragment is a DNA polymerase enzyme that lacks the 5' to 3' exonuclease activity of intact DNA Polymerase I, but does exhibit the 5' to 3' DNA polymerase and 3' to 5' exonuclease activities.
Applications: Fill-in of 5´ overhangs (1). Synthesis of probes by random primers labeling method (2). Sequencing single- and double-stranded DNA (3). Site-directed mutagenesis.
Source: Purified from E. coli expressing the Klenow fragment on a plasmid.
Performance and Quality Testing: SDS-PAGE purity; single-stranded and double-stranded endodeoxyribonuclease and self-priming assays; performance evaluated in fill-in reaction.
Unit Definition: One unit incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template•primer.
For Research Use Only. Not for use in diagnostic procedures.
規格
DescriptionDNA Polymerase
PolymeraseDNA Polymerase I
Exonuclease Activity3' to 5'
Quantity500 Units
Shipping ConditionApproved for shipment on Wet or Dry Ice
Hot StartNo
Unit Size500 units
內容物與存放
DNA Polymerase I, Large (Klenow) Fragment is supplied with a vial of 10X REact™ 2 Buffer [500 mM Tris-HCl (pH 8.0), 100 mM MgCl2 , 500 mM NaCl], vial of dilution buffer. Store at -20°C.