Thermo Fisher Scientific Pierce His Tag Protein Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with His-tagged fusion proteins.

Features of His Tag Protein Interaction Pull-Down Kit:

• 6xHis pull-down (Product No. 21277)—purifies protein interactors of any His-tagged fusion protein
• Complete kits—provide all components and detailed protocol for purifying protein:protein interactions
• No special equipment needed—use common laboratory equipment and reagents (e.g., microcentrifuge)
• Convenient—microcentrifuge spin columns facilitate simple and efficient manipulation of agarose beads, including simple processing of multiple samples
• Flexible—instructions include protocols for use with bait and prey proteins expressed from a variety of sample types
• Less non-specific binding—Cobalt chelate resin is more specific for histidine-tagged fusion proteins than nickel resins, resulting in less non-specific binding
• Binding—Binds 10 to 25 mg of histidine-tagged fusion protein per mL of resin

Applications:

• Discover a new protein:protein interaction from a cell lysate
• Confirm a putative interaction from a cell lysate or with a previously purified protein
• Extract protein:protein interaction information from in vitro transcription/translation lysates

You provide the tagged fusion protein as the 'bait' and the cells expressing the putative protein interaction target ('prey'), and the Pull-Down Kits provide everything else: cell lysis buffer, microcentrifuge spin columns, tag-specific affinity resin (agarose beads) and optimized buffers and protocol. The Pull-Down Kits are designed to teach the method to first-time users and to increase ease-of-use, convenience and reproducibility for experienced researchers.

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