Gateway™ entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway™ entry clone.深入閱讀
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產品號碼 A10462
價格 (TWD)/ 10 µg
價格9,930.00
線上優惠6,960.00僅限網絡訂單。不包括供應中心
(ends 01-Jan-2025)
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新增至購物車
價格 (TWD)/ 10 µg
線上優惠6,960.00僅限網絡訂單。不包括供應中心
(ends 01-Jan-2025)
新增至購物車
Gateway™ pENTR™ 1A Dual Selection Vector
產品號碼A10462
價格 (TWD)/ 10 µg
線上優惠6,960.00僅限網絡訂單。不包括供應中心
(ends 01-Jan-2025)
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新增至購物車
Gateway™ entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway™ entry clone. The entry clone is ready for recombination with a destination vector to create an expression clone. New: pENTR™ Dual Selection vectors!
The Gateway™ entry vectors (Table 1) offer the following: • attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway™ destination vector to ensure cloning of the gene of interest in the correct orientation for expression • Kozak consensus sequence for efficient translation initiation in eukaryotic systems • Ribosome binding site for efficient translation initiation in prokaryotic systems (pENTR™ 1A Dual Selection, pENTR™3C Dual Selection, and pENTR™11 Dual Selection vectors only) • rrnB transcription termination sequences to prevent basal expression of the PCR product of interest in E. coli • pUC origin for high-copy replication and maintenance of the plasmid in E. coli • Kanamycin resistance gene for selection in E. coli • The ccdB⁄chloramphenicol fusion gene located between the two attL sites for o negative selection and o Chloramphenicol selection in E. coli • Kanamycin resistance gene for selection in E. coli
For Research Use Only. Not for use in diagnostic procedures.