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The Jump-In™ Fast Gateway™ Core Kit is intended for rapid engineering of mammalian cell lines through the stable and irreversible integration of your gene of interest (GOI) at specific genomic loci (pseudo attP sites). Following stable integration, only 5-10 clones need to be analyzed to identify clones with high expression levels.
The Jump-In™ Fast Gateway™ Core Kit contains the promoter-less pJTI™ Fast DEST expression vector and a vector for transient expression of the PhiC31 integrase.
Please note: the pJTI™ Fast DEST expression vector requires the cloning of a promoter upstream of the gene of interest, which is facilitated by any of the MultiSite Gateway™ Pro Kits.
This kit is also a component of the Jump-In™ Fast Gateway™ System.
The Jump-In™ Fast Gateway™ Core Kit contains the promoter-less pJTI™ Fast DEST expression vector and a vector for transient expression of the PhiC31 integrase.
Please note: the pJTI™ Fast DEST expression vector requires the cloning of a promoter upstream of the gene of interest, which is facilitated by any of the MultiSite Gateway™ Pro Kits.
This kit is also a component of the Jump-In™ Fast Gateway™ System.
For Research Use Only. Not for use in diagnostic procedures.
規格
Cloning Method
Gateway™
Delivery Type
Transfection
Cell Type
Mammalian
Format
Kit
Promoter
None (Promoterless)
Vector
Jump-In Vectors, pJTI
Expression System
Mammalian
For Use With (Application)
Transfection
High-throughput Compatibility
High-throughput Compatible
Key Functions
Stable Cell Line Development, Targeted Integration, Clone your own Promoter
Product Line
Jump-In™
Product Type
Core Kit
Protein Tag
Untagged
Quantity
1 Kit
Selection Agent (Eukaryotic)
Hygromycin
Selection Marker Eukaryotic
HygroR
內容物與存放
The Jump-In™ Fast Gateway™ Core Kit is composed of 2 vectors:
pJTI™ Fast DEST Vector (150 ng⁄μl; total 40 μl),
pJTI™ PhiC31 Int Vector (0.5 μg⁄μl; total 20 μl).
Store vectors at -20°C.
pJTI™ Fast DEST Vector (150 ng⁄μl; total 40 μl),
pJTI™ PhiC31 Int Vector (0.5 μg⁄μl; total 20 μl).
Store vectors at -20°C.
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