The Applied Biosystems TaqMan SCID/SMA Assay is a multiplex real-time PCR assay that detects the SMN1 gene and TREC, as well as the RNase P gene as an internal genomic control. For an assay that also detects KREC, please see the TaqMAN SCID/SMA Plus Assay (Cat. No. A48566).
Features of the TaqMan SCID/SMA Assay include: • Optimized for SCID and SMA • Direct extraction from DBS samples for reduced hands-on time and cost • Compatible with 96- or 384-reaction protocols • High-quality results for 96 samples within 55 min using the fast cycling protocol • Minimal liquid-dispensing steps for easy automation
Complete solution from DBS to result The TaqMan SCID/SMA Assay detects SMN1, TREC, as well as the RNase P gene as an internal genomic control. The assay solution contains a mixture of primers and probes for each target plus an SMN2 blocker. From DBS to result, less than 40 min of hands-on time and only four reagent-dispensing steps are required for 96-well plates. One additional pipetting transfer step from DBS lysate extraction plate to qPCR plate is needed. A real-time PCR instrument with at least five filters is required to detect the reporter dyes plus the passive reference dye.
The TaqMan SCID/SMA Assay is a multiplex real-time PCR assay that detects exon 7 of SMN1 and TREC. The assay includes the RNase P gene as an internal amplification control, verifying successful sample extraction. The assay is optimized for high specificity to SMN1, eliminating cross-detection of SMN2.
For Research Use Only. Not for use in diagnostic procedures.
規格
Detection Method
Primer-probe
For Use With (Equipment)
QuantStudio™ 12 k Flex, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, QuantStudio™ Dx System (in RUO Mode)
Product Line
TaqMan™
Shipping Condition
Dry Ice
No. of Reactions
20,000 Reactions
Sample Type
DNA (Genomic), cDNA
內容物與存放
• DNA Extract All Reagents Kit • TaqPath ProAmp Multiplex Master Mix • TaqMan SCID/SMA Assay
Upon receipt, store at -20°C. After opening, store at 4°C.