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The RNAqueous™-96 Kit is used for high-throughput, phenol-free total RNA isolation from 100–2,000,000 cells or 0.1–1.5 mg of tissue using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The kit contains sufficient reagents for 192 (2 x 96) RNA purifications. The RNAqueous™-96 Kit is ideal for the isolation of total RNA from multiple samples and very small samples.
Using the RNAqueous™-96 Kit
The procedure uses an RNA-binding glass-fiber filter and either vacuum filtration or centrifugation methods to provide high yields of intact RNA. An optional on-the-filter DNase treatment can be performed to ensure removal of genomic DNA for RT-PCR applications. The 96-well plates are compatible with most common 96-well plate vacuum manifolds and 96-well plate centrifuge rotors. The RNAqueous™-96 Kit provides consistently high yields of RNA from various amounts of starting material. The high quality of RNA isolated is illustrated (see figure).
Accessory product
The RNAqueous™-96 Automated Kit (Cat. No. AM1812) is the same product in a format suitable for automation.
Using the RNAqueous™-96 Kit
The procedure uses an RNA-binding glass-fiber filter and either vacuum filtration or centrifugation methods to provide high yields of intact RNA. An optional on-the-filter DNase treatment can be performed to ensure removal of genomic DNA for RT-PCR applications. The 96-well plates are compatible with most common 96-well plate vacuum manifolds and 96-well plate centrifuge rotors. The RNAqueous™-96 Kit provides consistently high yields of RNA from various amounts of starting material. The high quality of RNA isolated is illustrated (see figure).
Accessory product
The RNAqueous™-96 Automated Kit (Cat. No. AM1812) is the same product in a format suitable for automation.
For Research Use Only. Not for use in diagnostic procedures.
規格
Isolation Technology
Silica Plate
Elution Volume
≥50 μL
Sample Type
Cells, Tissue
Final Product Type
Total RNA
High-throughput Compatibility
High-throughput Compatible
For Use With (Equipment)
MultiPROBE™ II HT
For Use With (Application)
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Quantity
192 Preps
Shipping Condition
Box 1: Dry Ice
Box 2: Room Temperature
Box 2: Room Temperature
Starting Material Amount
Cells: ≤106
Tissue: ≤1.5 mg
Tissue: ≤1.5 mg
內容物與存放
• 50 mL Nuclease-free Water; store at room temperature
• 50 mL Elution Solution; room temperature
• 2 Filter Plates; room temperature
• 2 Collection Plates; room temperature
• 2 Adhesive Plate Sealers; room temperature
• 2 Plate Lids; room temperature
• 50 mL Lysis/Binding Solution; 4°C
• 175 mL Wash Solution (concentrated); 4°C
• 53 mL Rebinding Mix; 4°C
• 12 mL DNase I Buffer; -20°C
• 1.25 mL DNase I; -20°C
• 50 mL Elution Solution; room temperature
• 2 Filter Plates; room temperature
• 2 Collection Plates; room temperature
• 2 Adhesive Plate Sealers; room temperature
• 2 Plate Lids; room temperature
• 50 mL Lysis/Binding Solution; 4°C
• 175 mL Wash Solution (concentrated); 4°C
• 53 mL Rebinding Mix; 4°C
• 12 mL DNase I Buffer; -20°C
• 1.25 mL DNase I; -20°C
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