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The RNAqueous™-Micro Kit is used for phenol-free total RNA isolation from micro-sized samples (e.g., from less than 500,000 cultured cells, from few as 10 laser capture microdissected cells, or from ≤10 mg of tissue) using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-free™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR. This kit provides:
• Highly concentrated RNA—for increased sensitivity in downstream assays
• Isolation of high-quality RNA from micro-scale samples
• Reagents/protocol for RNA recovery from LCM samples
How it works
Samples are solubilized in a powerful chaotropic solution, and RNA is purified on a glass fiber filter cartridge specifically designed for very small samples. A simple modification of the protocol allows recovery of both large and small RNA species including tRNAs, 5S rRNA, and microRNAs. The RNA is eluted in a minimal volume of 20 μL. Residual genomic DNA is efficiently removed using the DNA-free™ system included in the kit. The RNAqueous™-Micro Kit contains enough reagents for 50 RNA isolations of DNA-free RNA.
• Highly concentrated RNA—for increased sensitivity in downstream assays
• Isolation of high-quality RNA from micro-scale samples
• Reagents/protocol for RNA recovery from LCM samples
How it works
Samples are solubilized in a powerful chaotropic solution, and RNA is purified on a glass fiber filter cartridge specifically designed for very small samples. A simple modification of the protocol allows recovery of both large and small RNA species including tRNAs, 5S rRNA, and microRNAs. The RNA is eluted in a minimal volume of 20 μL. Residual genomic DNA is efficiently removed using the DNA-free™ system included in the kit. The RNAqueous™-Micro Kit contains enough reagents for 50 RNA isolations of DNA-free RNA.
For Research Use Only. Not for use in diagnostic procedures.
規格
Isolation Technology
Silica Spin Column
Purification Time
15 min. (After sample homogenization)
Elution Volume
10–20 μL
Sample Type
Cells, Tissue, Laser Capture Microdissection (LCM)
Final Product Type
Total RNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
For Use With (Application)
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Quantity
50 Preps
Shipping Condition
Box 1: Dry Ice
Box 2: Room Temperature
Box 2: Room Temperature
Starting Material Amount
Cells: ≤105
Tissue: ≤10 mg
Tissue: ≤10 mg
內容物與存放
• 50 Filter Cartridges with Collection Tubes; store at room temperature
• 50 Elution Tubes (1.5 mL); room temperature
• 15 mL Wash Solution 1 (concentrated); room temperature
• 28 mL Wash Solution 2/3 (concentrated); 4°C
• 25 mL Lysis Solution; 4°C
• 200 μL LCM Additive; -20°C
• 55 μL DNase; -20°C
• 600 μL 10X DNase I Buffer; -20°C
• 150 μL DNase Inactivation Reagent; -20°C
• 5 mL Elution Solution; room temperature
• 50 Elution Tubes (1.5 mL); room temperature
• 15 mL Wash Solution 1 (concentrated); room temperature
• 28 mL Wash Solution 2/3 (concentrated); 4°C
• 25 mL Lysis Solution; 4°C
• 200 μL LCM Additive; -20°C
• 55 μL DNase; -20°C
• 600 μL 10X DNase I Buffer; -20°C
• 150 μL DNase Inactivation Reagent; -20°C
• 5 mL Elution Solution; room temperature
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