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產品號碼 | 包裝規格 | 價格 (TWD) | 供應狀況 | 數量 | |
---|---|---|---|---|---|
EP0441 | 10,000 units | 價格 3,460.00 線上優惠 3,182.00 (01-Oct-2024 結束) 您的價格 | - | ||
EP0442 | 5 x 10,000 units | 價格 16,800.00 線上優惠 11,760.00 (01-Oct-2024 結束) 您的價格 | - |
Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids that is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.
Features of RevertAid Reverse Transcriptase include:
• Efficient synthesis of full-length first strand cDNA up to 13 kb
• Optimum activity at 42°C
• Active up to 50°C
• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, and fluorescein-labeled nucleotides)
Applications
• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• DNA labeling
• Analysis of RNA by primer extension
Source
RevertAid Reverse Transcriptase is sourced from E.coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase.
• RevertAid Reverse Transcriptase (200 U/μL)
• 5X Reaction Buffer
Store at –20°C.