Thermo Scientific Phusion Human Specimen Direct PCR Kit delivers unprecedented convenience for DNA amplification by allowing PCR directly from unpurified human samples. A tiny amount of sample is used directly in the PCR reaction with no prior purification steps. This allows significant savings in both time and cost.

Tested sample materials include buccal swabs, saliva, amniotic fluid, hair, fingernails, teeth, and skin biopsies. The kit is compatible with fresh, frozen, and even Formalin-Fixed, Paraffin Embedded (FFPE) human tissue samples. It includes optimized reagents and detailed protocols for many sample materials. The modified Phusion Hot Start II High-Fidelity DNA Polymerase utilized in the kit is a highly accurate proofreading DNA polymerase; PCR products obtained are perfectly suitable even for cloning purposes.

Highlights

• No need for time-consuming and expensive DNA purification steps
• Two simple protocols for various applications
• Extremely short PCR protocol times
• Modified Phusion Hot Start II High-Fidelity DNA Polymerase delivers abundant yields of specific product

The optimal annealing temperature for Phusion DNA Polymerases may differ significantly from that of Taq-based DNA polymerases.

For optimal results start by accurately calculating your Tm with our Tm calculator.