Applications
4-Methylumbelliferyl sulfate potassium salt is a fluorometric substrate for the determination of sulfatase. This substrate has been used in the discovery of tyrosine sulfotransferase inhibitors, particularly in P-selectin glycoprotein ligand-1 binding. This compound is virtually non-fluorescent until cleavage by sulfatase enzymes, then emits blue fluorescence (450 nm) with excitation at 360 nm. Use of this compound in high-throughput fluorescence based screening led to the discovery of several potent beta-arylsulfotransferase IV (beta-AST-IV) inhibitors.

Solubility
Soluble in water at 5mg/ml

Notes
Excitation wavelength, 334 nm; emission wavelength, 370nm (pH 10.4). Reaction product: excitiation wavelength, 360nm; emission wavelength, 449nm