The pCR™8/GW/TOPO™ TA Cloning Kit delivers simple 5 minute cloning and access to the Gateway™ recombination cloning vector platform. Our pCR™8 TOPO™ TA vector contains a minimal MCS, so you sequence more of your insert and less of the vector.
With our patented att sites, the pCR™8 vector is the simplest way to create an Entry Clone, which facilitates easy access via Gateway™ recombination cloning to a variety of expression vectors without tedious sub-cloning, saving you valuable research time.
The pCR™8/GW/TOPO™ TA Cloning Kit offers:
• att sites for rapid recombination into a variety of Gateway™ destination vectors • 3´-T overhangs for direct ligation of Taq-amplified PCR products • Sequencing primer sites within 55 bp of insertion site ensures you sequence more insert, less vector • Spectinomycin resistance gene for robust selection in E. coli • EcoRI sites flanking the PCR product insertion site for easy excision of inserts
This kit includes One Shot™ TOP10 Chemically Competent E. coli.
For Research Use Only. Not for use in diagnostic procedures.
規格
Cloning Method
TOPO™ with Gateway™
No. of Reactions
20 reactions
Product Line
One Shot™
Product Type
TA Cloning Kit
Vector
pCR
Unit Size
20 reactions
內容物與存放
The pCR™8/GW/TOPO™ TA Cloning Kit contains the TOPO™-adapted, ready-to-use pCR™8/GW/TOPO™ Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers, One Shot™ TOP10 Chemically Competent E. coli Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid. Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.