內容物與存放
The pTrcHis- and pTrcHis2-TOPO™ TA Expression Kits each contain two boxes. The TOPO™ TA box contains 200 ng of linearized, topoisomerase I-activated pTrcHis-TOPO™ or pTrcHis2-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, a control template and primers, primers for sequencing or PCR screening, and an expression control plasmid. The One Shot™ TOP10 box contains twenty-one, 50-μl aliquots of chemically competent TOP10 E. coli, S.O.C. medium, and a supercoiled control plasmid. Store the TOPO TA Cloning™ box at -20°C. Store the One Shot™ box at -80°C. All reagents are guaranteed stable for 6 months when properly stored.