Thermo Scientific™ DreamTaq™ Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq™ Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs. The master mix allows easy reaction setup with fewer pipetting steps, with only primers, template, and water needing to be added. A version of this master mix is also available with pre-added density reagent and electrophoresis tracking dyes.

DreamTaq Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases. DreamTaq Hot Start DNA Polymerase employs antibody-based inhibition of DNA polymerase activity at ambient temperatures to prevent the amplification of non-specific products prior to the amplification step. With DreamTaq Hot Start DNA Polymerase, reactions can be set up at room temperature using the same protocol and cycling conditions as conventional Taq DNA polymerases.

DreamTaq Hot Start PCR Master Mix is formulated to enhance productivity:

• Better reaction outcomes
  • Higher yield of target amplicons from low template amounts
  • Increased reaction specificity due to reliable hot-start technology
  • Wider range of amplicon lengths—routine amplification of genomic DNA fragments up to 6kb
• Enhanced convenience
  • Room temperature reaction set-up
  • Minimized optimization of Mg²⁺ concentration and of primer annealing temperatures due to optimized reaction buffer
  • Simplified workflow and minimized pipetting steps with 2X master mix format

Applications

• Routine PCR
• Colony PCR
• Genotyping
• RT-PCR
• Generation of PCR products for TA cloning