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The Neon™ Transfection System 100 μL Kit is designed specifically for use with the Neon™ Transfection System. Use this kit for transfection volumes of 100 μL, containing 5 × 105–2 × 106 adherent cells or 1 × 106–5 × 106 suspension cells. Cells that have been transfected using the included 100 μL Neon™ Tips are then ready to be washed and plated into a 6-well, 60 mm, or 10 cm culture dish, depending on your protocol.
For Research Use Only. Not for use in diagnostic procedures.
規格
Classification
Animal Origin-Free, Chemically Defined, Low-Protein, Xeno-Free, Reduced Growth Factors, Reduced Serum
Cell Type
Stem Cells, Primary Cells, Hard-to-Transfect Cells
Format
6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample Type
Plasmid DNA, Synthetic siRNA
Promoter
CMV
Transfection Technique
Electroporation
For Use With (Equipment)
Neon™ Transfection System
For Use With (Application)
Transfection
No. of Reactions
50 Reactions
Product Line
Neon™
Product Type
Transfection System
Quantity
25 x 2 Reactions
Serum Compatible
Yes
Shipping Condition
Room Temperature
Starting Material Amount
2x10^4 - 6 x10^8 cells; Varies by cell type
System Type
Neon™ Transfection System
Volume (Metric) Sample
100μl
內容物與存放
The Neon™ Transfection System 100 μL Kit comes with 10 mL resuspension buffer R, 10 mL resuspension buffer T, and 75 mL E2 electrolytic buffer in one box and 25 x 100μL reaction tips and 5 electroporation tubes in a separate box. Refer to the Neon™ Transfection System users manual for protocol instructions. Store the buffer box at 4°C and the tips & tubes box at room temperature.
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