Achieve faster staining and more accurate results without the need for wash steps or quencher dye with pH-sensitive pHrodo Green E. coli BioParticles Conjugate for Phagocytosis. This dye-bioparticle conjugate provides faster and more accurate results than any other dye-bioparticle conjugate and has reduced signal variability and improved timing in sensitive experiments. pHrodo Green dye conjugates are non-fluorescent outside the cell, but fluoresce bright green in phagosomes, specifically detecting intracellular phagocytosis and endocytosis. The pHrodo Green E. coli BioParticles Conjugate can also be multiplexed with a wide variety of blue, red, and far-red dyes reporters such as Mitosox Red, CellEvent Caspase 3/7 Red, NucBlue, RFPs, and Mitotracker Deep Red, among many others.

The fluorescence of the novel pHrodo Green dye dramatically increases as pH decreases from neutral to acidic, making it an ideal tool to study phagocytosis and its regulation by drugs and/or environmental factors. The lack of fluorescence outside the cell eliminates the need for wash steps and quencher dyes.

Use the ready-made pHrodo Green E. coli BioParticles Conjugate in imaging, high content screening, high throughput screening, and flow applications. Enough reagent is provided to perform 100 tests when using 100 μL in each well of a 96-well plate. The pHrodo Green dye is also available as a conjugate of zymosan BioParticles, S. aureus BioParticles, or dextran 10,000 MW. To create other conjugates, such as antibody conjugates, use pHrodo Green STP ester or pHrodo Green maleimide.

See User Manual for solubility instructions.