Qubit™ microRNA Assay Kits
Qubit™ microRNA Assay Kits
Invitrogen™

Qubit™ microRNA Assay Kits

Achieve accurate miRNA quantitation, even in the presence of rRNA, large mRNA, or contaminants, with the Qubit microRNA Assay Kit, which offers 100 and 500 assay formats.
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優惠代碼:P5780456
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產品號碼No. of Reactions
Q32880Promo Image100 Reactions
Q32881Promo Image500 Reactions
產品號碼 Q32880
價格 (TWD)
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新增至購物車
No. of Reactions:
100 Reactions
價格 (TWD)
***
新增至購物車
Achieve accurate and precise quantification of small RNA (∼20 nucleotides or base pairs) with Qubit miRNA (microRNA) Assay Kits. These miRNA quantification kits enable quick detection of all types of small RNA, including microRNA and siRNA, and both single stranded and double stranded RNA. The assay is highly selective for small RNA over rRNA or large mRNA, and can tolerate contaminants such as salts, solvents, or detergents.
The Qubit miRNA Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of microRNA samples. This assay is highly selective for miRNA over rRNA or large mRNA (>1000 bp). Depending on sample volume, the assay kit is designed to be accurate for initial miRNA sample concentrations of 0.025 to 150 ng/μL, providing a detection range of 0.5−150 ng.

The Qubit microRNA Assay Kit provides concentrated assay reagent, dilution buffer, and pre-diluted miRNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 μL and 20 μL is acceptable), and read the concentration using the Qubit Fluorometer.

Notes
• Use with thin-wall, clear, 0.5-mL PCR tubes (Cat. No. Q32856) for the Qubit 4 Fluorometer and 8 x 200-μL tube strips (Cat. No. Q33252) for the Qubit Flex Fluorometer

For Research Use Only. Not for use in diagnostic procedures.
規格
Detection MethodFluorescence
Excitation/Emission498/518
For Use With (Application)microRNA Quantitation
For Use With (Equipment)Qubit Fluorometer
No. of Reactions100 Reactions
Product LineQubit
Product TypemicroRNA Assay Kit
Quantity100 assays
Shipping ConditionRoom Temperature
Unit SizeEach

Enhance your Qubit Fluorometer with the latest firmware

New Qubit 4 and Qubit Flex firmware features include importing sample IDs, renaming files before exporting, a countdown timer, and on-board troubleshooting guidance. Get the most out of your Qubit assays with these powerful new tools.

Download the latest Qubit firmware ›

常見問答集 (常見問題)

Can the Quant-iT RNA assay be used to quantitate RNA labeled with digoxigenin?

Yes, the tag does not interfere with the signal.

What are the excitation/emission wavelengths for dyes in the Qubit Assays?

The exact excitation/emission wavelength information is proprietary. Here are the approximate excitation/emission wavelengths:

- Qubit dsDNA HS Assay: ~500 nm/ ~530 nm
- Qubit dsDNA BR Assay: ~510 nm/ ~530 nm
- Qubit ssDNA Assay: ~490 nm/ ~520 nm
- Qubit RNA HS Assay: ~640 nm/ ~670 nm
- Qubit RNA BR Assay: ~640 nm/ ~670 nm
- Qubit microRNA Assay: ~500 nm/ ~520 nm
- Qubit Protein Assay: ~470 nm/ ~570 nm

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

How does the accuracy and sensitivity of the Qubit quantitation assays using the Qubit fluorometer compare to a microplate reader?

The accuracy and sensitivity of the Qubit quantitation assays are the same as that of a microplate reader. This was a requirement during product development. The detection limits for each Qubit kit can be found on the corresponding product manual, which can be found by searching our website by keyword or catalog number.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

Can the Qubit kits give an indication of sample quality in nucleic acid samples?

No. The Qubit DNA and RNA kits only quantify the amount of either DNA or RNA in the sample. The Qubit fluorometer cannot take absorbance readings to provide a A260/A280 ratio or detect protein in nucleic acid samples. This can be done with the NanoDrop instrument. If your sample contains protein or other contaminants that can affect the assay, it should be further purified.

If your sample may contain both DNA and RNA, one may use either (or both) the DNA and RNA Qubit kits and compare with samples treated with either RNase or DNase to get an accurate determination of DNA or RNA, respectively.

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