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The pLenti6.2⁄V5-DEST™ Gateway™ Vector is a Gateway™-adapted ViraPower™ II lentiviral expression vector for lentiviral-based expression of a target gene in dividing and non-dividing mammalian cells. The vector has the CMV promoter for driving constitutive expression of the target gene and the PGK promoter for driving long-term, persistent expression of the Blasticidin stable selection marker.
Advantages
• Stable expression
• Long-term experiments
• High-throughput screening
• Accurate titer of functional virus (using Blasticidin method)
• Flexible and versatile Gateway™ recombination cloning technology
Key Features
• Human cytomegalovirus (CMV) immediate early promoter to control high-level expression of the gene of interest
• PKG Promoter for expression of Blasticidin selection marker
Kit includes
• pLenti6.2⁄V5-DEST™ Gateway™ Vector box
• One Shot™ Stbl3™ chemically competent E. coli (C7373-03)
Related SKUs
• 293FT Cell Line (R70007; R70007)
• ViraPower™ Lentiviral Support Kit (K4970-00)
• ViraPower™ Lentiviral Packaging Mix (K4975-00)
• Lipofectamine™ 2000 (11668-019; 11668-027)
For research use only. Not intended for any therapeutic or diagnostic use.
Advantages
• Stable expression
• Long-term experiments
• High-throughput screening
• Accurate titer of functional virus (using Blasticidin method)
• Flexible and versatile Gateway™ recombination cloning technology
Key Features
• Human cytomegalovirus (CMV) immediate early promoter to control high-level expression of the gene of interest
• PKG Promoter for expression of Blasticidin selection marker
Kit includes
• pLenti6.2⁄V5-DEST™ Gateway™ Vector box
• One Shot™ Stbl3™ chemically competent E. coli (C7373-03)
Related SKUs
• 293FT Cell Line (R70007; R70007)
• ViraPower™ Lentiviral Support Kit (K4970-00)
• ViraPower™ Lentiviral Packaging Mix (K4975-00)
• Lipofectamine™ 2000 (11668-019; 11668-027)
For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
規格
Constitutive or Inducible System
Constitutive
Delivery Type
Lentiviral
Promoter
CMV
Product Type
Lentiviral Expression Vector
Selection Agent (Eukaryotic)
Blasticidin
Selection Marker Promoter
PGK Promoter
Protein Tag
V5 Epitope Tag
Cloning Method
Gateway
Vector
pLenti
Product Line
Gateway™, ViraPower™
For Use With (Application)
Viral Expression
內容物與存放
pLenti6.2⁄V5-DEST™ Gateway™ Vector Box contains pLenti6.2⁄V5-DEST, 40 μl of 150 ng⁄μl vector in 10 mM Tris-HCl, 1mM EDTA, pH 8.0 and pLenti6.2⁄V5-GW⁄lacZ control, 20 μl of 0.5 μg⁄μl vector in 10 mM Tris-HCl, 1mM EDTA, pH 8.0. Store at –20°C.
One Shot™ Stbl3™ chemically competent E. coli box contains transformation reagents including 20 single-use 50 μl aliquots of One Shot™ Competent Cells, S.O.C. medium, and pUC19 supercoiled plasmid control. Store at -80°C.
One Shot™ Stbl3™ chemically competent E. coli box contains transformation reagents including 20 single-use 50 μl aliquots of One Shot™ Competent Cells, S.O.C. medium, and pUC19 supercoiled plasmid control. Store at -80°C.
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