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The InsectSelect™ Kit features the pIZ/V5-His vector for high-level expression in a variety of insect cells. This exceptionally small vector has several features that facilitate expression, analysis, and detection of recombinant protein in insect cells:
• The OpIE2 promoter for constitutive expression
• The Zeocin™ resistance gene for rapid selection of stably transfected cell lines
• C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification using nickel-chelating resin
• The OpIE2 promoter for constitutive expression
• The Zeocin™ resistance gene for rapid selection of stably transfected cell lines
• C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification using nickel-chelating resin
For Research Use Only. Not for use in diagnostic procedures.
規格
Promoter
OplE2
Product Type
Insect Cell Expression Vector
Protein Tag Position (to your gene)
C-terminal
Protein Tag
His Tag (6x), V5 Epitope Tag
Cloning Method
Restriction Enzyme/MCS
Vector
pIZ
Product Line
InsectSelect™
內容物與存放
The InsectSelect™ Kit includes 20 μg each of supercoiled, lyophilized pIZ/V5-His and pIZ/V5-His/CAT, 2 μg of lyophilized OpIE2 Reverse Sequencing primer, 1 g Zeocin™, Cellfectin™ Reagent, and 1 L of medium for the insect cell line chosen. Sf9 cells are supplied with GIBCO™ Grace's Insect Media, High Five™ Cells are supplied with GIBCO™ Express Five™ Serum-Free Medium. The pIZ/V5-His Vector Kit contains only the expression vectors and primer as described above. Store frozen insect cells in liquid nitrogen. Store medium and Cellfectin™ Reagent at +4°C. Store all other reagents at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
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