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WesternBreeze® Chromogenic Kits detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots). Detection is accomplished with a ready-to-use BCIP/NBT substrate for alkaline phosphatase. Non-fading purple precipitates develop at the protein bands on the membrane. WesternBreeze® chromogenic offers:
• Clear background
• High sensitivity-low picogram levels detectable
• High specificity
• One simple protocol-no optimization required
• Clear background
• High sensitivity-low picogram levels detectable
• High specificity
• One simple protocol-no optimization required
For Research Use Only. Not for use in diagnostic procedures.
規格
Reactivity
Rabbit
Shipping Condition
Wet Ice
Substrate Type
AP (Alkaline Phosphatase) Substrate
Detection Method
Chromogenic
Membrane Compatibility
Nitrocellulose, PVDF
Product Line
WesternBreeze™
Product Type
Western Blot Kit
Cross Reactivity
Rabbit
內容物與存放
The WesternBreeze Chromogenic kits include blocking solutions, primary antibody diluent, ready-to-use secondary antibody solution (anti-mouse, anti-rabbit or anti-goat), ready-to-use chromogenic substrate, wash solutions, and incubation trays. Each kit contains complete reagents for 20 mini-blots.
Store the kits at 2–8°C. Guaranteed stable for 6 months when properly stored.
Store the kits at 2–8°C. Guaranteed stable for 6 months when properly stored.
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